验证数据展示
经过测试的应用
| Positive WB detected in | human kidney tissue, COLO 320 cells, HEK-293 cells, HepG2 cells, human brain tissue, mouse brain tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | mouse testis tissue, mouse brain tissue, mouse kidney tissue, human kidney tissue, human placenta tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Planning an IHC experiment? We recommend our IHCeasy PRR5 Ready-To-Use IHC Kit. PRR5 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2400 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IHC | See 1 publications below |
产品信息
17948-1-AP targets PRR5 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | IHC |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human |
| 免疫原 | PRR5 fusion protein Ag12424 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | proline rich 5 (renal) |
| 别名 | Proline-rich protein 5, Proline rich protein 5, proline rich 5 (renal), PP610, FLJ20185k |
| 计算分子量 | 31 kDa, 43 kDa |
| 观测分子量 | 33 kDa, 50 kDa |
| GenBank蛋白编号 | BC016921 |
| 基因名称 | PRR5 |
| Gene ID (NCBI) | 55615 |
| RRID | AB_2268865 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P85299 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PRR5, also named as PROTOR1 and PP610, is a subunit of mTORC2, which regulates cell growth and survival in response to hormonal signals. PRR5 plays an important role in regulation of PDGFRB expression and in modulation of platelet-derived growth factor signaling. It acts as a tumor suppressor in breast cancer. PRR5 has some isoforms with MW 31-33 kDa and 41-50 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PRR5 antibody 17948-1-AP | Download protocol |
| IHC protocol for PRR5 antibody 17948-1-AP | Download protocol |
| IF protocol for PRR5 antibody 17948-1-AP | Download protocol |
| IP protocol for PRR5 antibody 17948-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
