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PRR5 Polyclonal antibody

PRR5 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 17948-1-AP

Print datasheet

Synonyms

Proline-rich protein 5, Proline rich protein 5, proline rich 5 (renal), PP610, FLJ20185k

验证数据展示

  • IHC staining of mouse testis using 17948-1-AP

    IHC staining of mouse testis using 17948-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 17948-1-AP (PRR5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse testis using 17948-1-AP

    IHC staining of mouse testis using 17948-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 17948-1-AP (PRR5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of human kidney using 17948-1-AP

    WB analysis of human kidney using 17948-1-AP

    human kidney tissue were subjected to SDS PAGE followed by western blot with 17948-1-AP (PRR5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 17948-1-AP

    IF Staining of HepG2 using 17948-1-AP

    Immunofluorescent analysis of HepG2 cells, using PRR5 antibody 17948-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  • IP experiment of mouse brain using 17948-1-AP

    IP experiment of mouse brain using 17948-1-AP

    IP result of anti-PRR5 (IP:17948-1-AP, 3ug; Detection:17948-1-AP 1:300) with mouse brain tissue lysate 4000ug.

经过测试的应用

Positive WB detected inhuman kidney tissue, COLO 320 cells, HEK-293 cells, HepG2 cells, human brain tissue, mouse brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inmouse testis tissue, mouse brain tissue, mouse kidney tissue, human kidney tissue, human placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2400
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IHCSee 1 publications below

产品信息

17948-1-AP targets PRR5 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Cited ApplicationsIHC
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen PRR5 fusion protein Ag12424 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name proline rich 5 (renal)
Synonyms Proline-rich protein 5, Proline rich protein 5, proline rich 5 (renal), PP610, FLJ20185k
Calculated Molecular Weight 31 kDa, 43 kDa
Observed Molecular Weight 33 kDa, 50 kDa
GenBank Accession NumberBC016921
Gene Symbol PRR5
Gene ID (NCBI) 55615
RRIDAB_2268865
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP85299
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PRR5, also named as PROTOR1 and PP610, is a subunit of mTORC2, which regulates cell growth and survival in response to hormonal signals. PRR5 plays an important role in regulation of PDGFRB expression and in modulation of platelet-derived growth factor signaling. It acts as a tumor suppressor in breast cancer. PRR5 has some isoforms with MW 31-33 kDa and 41-50 kDa.

实验方案

Product Specific Protocols
WB protocol for PRR5 antibody 17948-1-APDownload protocol
IHC protocol for PRR5 antibody 17948-1-APDownload protocol
IF protocol for PRR5 antibody 17948-1-APDownload protocol
IP protocol for PRR5 antibody 17948-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Nat Cell Biol

m6A mRNA methylation regulates AKT activity to promote the proliferation and tumorigenicity of endometrial cancer.

Authors - Jun Liu
View Article