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PRRC1 Polyclonal antibody

PRRC1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 27887-1-AP

Print datasheet

Synonyms

proline rich coiled coil 1, Protein PRRC1, PRRC1

验证数据展示

  • WB analysis of HeLa using 27887-1-AP

    WB analysis of HeLa using 27887-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 27887-1-AP (PRRC1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • IHC staining of human pancreas using 27887-1-AP

    IHC staining of human pancreas using 27887-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 27887-1-AP (PRRC1 antibody) at dilution of 1:200 (under 10x lens).

经过测试的应用

Positive WB detected inHeLa cells, mouse heart tissue
Positive IHC detected inhuman pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27887-1-AP targets PRRC1 in WB, IHC, ELISA applications and shows reactivity with Human, Mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity Human, Mouse
Immunogen PRRC1 fusion protein Ag27349 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name proline-rich coiled-coil 1
Synonyms proline rich coiled coil 1, Protein PRRC1, PRRC1
Calculated Molecular Weight 445 aa, 47 kDa
Observed Molecular Weight 50 kDa
GenBank Accession NumberBC017066
Gene Symbol PRRC1
Gene ID (NCBI) 133619
RRIDAB_2881005
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96M27
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PRRC1 antibody 27887-1-APDownload protocol
IHC protocol for PRRC1 antibody 27887-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols