验证数据展示
经过测试的应用
| Positive WB detected in | mouse kidney tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
11618-1-AP targets PRRG2 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 | PRRG2 fusion protein Ag2197 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | proline rich Gla (G-carboxyglutamic acid) 2 |
| 别名 | Transmembrane gamma-carboxyglutamic acid protein 2, TMG2, Proline-rich Gla protein 2, Proline-rich gamma-carboxyglutamic acid protein 2, PRGP2 |
| 计算分子量 | 179 aa, 20 kDa |
| 观测分子量 | 16-22 kDa |
| GenBank蛋白编号 | BC026032 |
| 基因名称 | PRRG2 |
| Gene ID (NCBI) | 5639 |
| RRID | AB_2171684 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O14669 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Proline-rich Gla protein 2 (PRRG2) represents one of the four known transmembrane-carboxyglutamic acid proteins in vertebrates. As a founding member of the vitamin K-dependent, single-pass integral membrane protein family, PRRG2 is characterized by an extracellular N-terminus consisting of approximately 45 amino acids rich in Gla, as well as a conserved Pro/Leu-Pro-xaa-Tyr (PY) motif proximal to its C-terminus. PRRG2 primarily localizes to the cell surface, where the Gla domain is exposed extracellularly, serving as a pivotal gene node with positive autoregulatory function that plays a critical role in maintaining human hemostasis (PMID: 38227081). The calculated molecular weight of PRRG2 is usually approximately 22 kda.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PRRG2 antibody 11618-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |