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验证数据展示

  • IHC staining of mouse brain using 29790-1-AP

    IHC staining of mouse brain using 29790-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 29790-1-AP (PRSS1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 29790-1-AP

    IF Staining of HeLa using 29790-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using PRSS1 antibody (29790-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

Trypsin 1/PRSS1 Polyclonal antibody

Trypsin 1/PRSS1 Polyclonal Antibody for IHC, IF/ICC, ELISA
Cat No. 29790-1-AP

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, IF/ICC, ELISA

Synonyms

PRSS1, TRYP1, TRY1, TRP1, Beta trypsin

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29790-1-AP targets Trypsin 1/PRSS1 in IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications IHC, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen Trypsin 1/PRSS1 fusion protein Ag31383 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name protease, serine, 1 (trypsin 1)
Synonyms PRSS1, TRYP1, TRY1, TRP1, Beta trypsin
Calculated Molecular Weight 27 kDa
GenBank Accession NumberBC128226
Gene Symbol PRSS1
Gene ID (NCBI) 5644
RRIDAB_3669696
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP07477
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PRSS1 encodes a trypsinogen, which is a member of the trypsin family of serine proteases. This enzyme is secreted by the pancreas and cleaved to its active form in the small intestine. It is active on peptide linkages involving the carboxyl group of lysine or arginine. Mutations in this gene are associated with hereditary pancreatitis. This gene and several other trypsinogen genes are localized to the T cell receptor beta locus on chromosome 7.

实验方案

Product Specific Protocols
IHC protocol for Trypsin 1/PRSS1 antibody 29790-1-APDownload protocol
IF protocol for Trypsin 1/PRSS1 antibody 29790-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols