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验证数据展示

  • WB analysis of SW 1990 using 19515-1-AP

    WB analysis of SW 1990 using 19515-1-AP

    SW 1990 cells were subjected to SDS PAGE followed by western blot with 19515-1-AP (PRSS27 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IP experiment of SW 1990 using 19515-1-AP

    IP experiment of SW 1990 using 19515-1-AP

    IP result of anti-PRSS27 (IP:19515-1-AP, 4ug; Detection:19515-1-AP 1:300) with SW 1990 cells lysate 2960ug.

  • IHC staining of human pancreas using 19515-1-AP

    IHC staining of human pancreas using 19515-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 19515-1-AP (PRSS27 Antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human pancreas using 19515-1-AP

    IHC staining of human pancreas using 19515-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 19515-1-AP (PRSS27 Antibody) at dilution of 1:200 (under 40x lens).

PRSS27 Polyclonal antibody

PRSS27 Polyclonal Antibody for WB, IP, IHC, ELISA
Cat No. 19515-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IP, IHC, ELISA

别名

CAPH2, Marapsin, MPN, Pancreasin, protease, serine 27, PRSS27, Serine protease 27

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inSW 1990 cells
Positive IP detected inSW 1990 cells
Positive IHC detected inhuman pancreas tissue, human oesophagus cancer tissue, mouse pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

19515-1-AP targets PRSS27 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IP, IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 protease, serine 27
别名 CAPH2, Marapsin, MPN, Pancreasin, protease, serine 27, PRSS27, Serine protease 27
计算分子量 32 kDa
观测分子量 28 kDa
GenBank蛋白编号NM_031948
基因名称 PRSS27
Gene ID (NCBI) 83886
RRIDAB_2878585
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9BQR3
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PRSS27, also named as CAPH2, MPN, belongs to the peptidase S1 family. The trypsin-like serine protease marapsin (Prss27) is a member of the large protease gene cluster at human chromosome 16p13.3. The Prss27 gene is expressed by a variety of human, mouse, and opossum tissues. In humans and mice, it is especially abundant in stratified squamous epithelia of cornea, larynx, esophagus, and cervix and is induced in wounded and psoriatic skin (PMID: 23447538). Prss27 expression was restricted to tissues containing stratified squamous epithelia and was absent or only weakly expressed in all other tissues, including the pancreas.

实验方案

Product Specific Protocols
WB protocol for PRSS27 antibody 19515-1-APDownload protocol
IHC protocol for PRSS27 antibody 19515-1-APDownload protocol
IP protocol for PRSS27 antibody 19515-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols