验证数据展示
经过测试的应用
| Positive WB detected in | human colon tissue, mouse kidney tissue |
| Positive IHC detected in | mouse ovary tissue, human ovary tumor tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 11 publications below |
| IHC | See 5 publications below |
产品信息
15527-1-AP targets PRSS8 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | PRSS8 fusion protein Ag7234 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | protease, serine, 8 |
| 别名 | Prostasin light chain, Prostasin heavy chain, EC:3.4.21.-, Channel-activating protease 1, CAP1 |
| 计算分子量 | 36 kDa |
| 观测分子量 | 39 kDa |
| GenBank蛋白编号 | BC001462 |
| 基因名称 | PRSS8 |
| Gene ID (NCBI) | 5652 |
| RRID | AB_2172168 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q16651 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Protease serine S1 family member 8 (PRSS8), a membrane-anchored serine protease, has been reported to be involved in the development of several human cancers. PRSS8 protein level was highly expressed in well differentiated cancer cell and was lower or absent in poorly differentiated cancer cell. Significantly higher levels of prostasin were found in early stage OVC serum samples compared to benign ovarian and normal donor samples. Overexpression of PRSS8 mRNA and high levels of prostasin in multiple subtypes of early stage ovarian tumors may provide clinical biomarkers for early detection of OVC. (PMID: 27036110, PMID: 27050145)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PRSS8 antibody 15527-1-AP | Download protocol |
| IHC protocol for PRSS8 antibody 15527-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Acta Physiol (Oxf) Zymogen-locked mutant prostasin (Prss8) leads to incomplete proteolytic activation of the epithelial sodium channel (ENaC) and severely compromises triamterene tolerance in mice. | ||
J Hypertens Prostasin and matriptase (ST14) in placenta from preeclamptic and healthy pregnant women. | ||
Acta Physiol (Oxf) Apical serine protease activity is necessary for assembly of a high-resistance renal collecting duct epithelium. | ||
Pflugers Arch The epithelial Na+ channel α- and γ-subunits are cleaved at predicted furin-cleavage sites, glycosylated and membrane associated in human kidney. | ||
