PRSS8 Polyclonal antibody
PRSS8 Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | human colon tissue, mouse kidney tissue |
Positive IHC detected in | mouse ovary tissue, human ovary tumor tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:1000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 11 publications below |
IHC | See 5 publications below |
产品信息
15527-1-AP targets PRSS8 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | WB, IHC, ELISA Application Description |
Cited Applications | WB, IHC |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse |
Immunogen | PRSS8 fusion protein Ag7234 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | protease, serine, 8 |
Synonyms | Prostasin light chain, Prostasin heavy chain, EC:3.4.21.-, Channel-activating protease 1, CAP1 |
Calculated Molecular Weight | 36 kDa |
Observed Molecular Weight | 39 kDa |
GenBank Accession Number | BC001462 |
Gene Symbol | PRSS8 |
Gene ID (NCBI) | 5652 |
RRID | AB_2172168 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q16651 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for PRSS8 antibody 15527-1-AP | Download protocol |
IHC protocol for PRSS8 antibody 15527-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Acta Physiol (Oxf) Zymogen-locked mutant prostasin (Prss8) leads to incomplete proteolytic activation of the epithelial sodium channel (ENaC) and severely compromises triamterene tolerance in mice. | ||
J Hypertens Prostasin and matriptase (ST14) in placenta from preeclamptic and healthy pregnant women. | ||
Acta Physiol (Oxf) Apical serine protease activity is necessary for assembly of a high-resistance renal collecting duct epithelium. | ||
Pflugers Arch The epithelial Na+ channel α- and γ-subunits are cleaved at predicted furin-cleavage sites, glycosylated and membrane associated in human kidney. | ||