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PRTN3 Polyclonal antibody

PRTN3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 25278-1-AP

Print datasheet

Synonyms

ACPA, AGP7, C ANCA, C ANCA antigen, Leukocyte proteinase 3, MBN, MBT, Myeloblastin, Neutrophil proteinase 4, P29, PR 3, PR3, proteinase 3, PRTN3, Wegener autoantigen

验证数据展示

  • WB analysis of human placenta using 25278-1-AP

    WB analysis of human placenta using 25278-1-AP

    human placenta tissue were subjected to SDS PAGE followed by western blot with 25278-1-AP (PRTN3 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human tonsillitis using 25278-1-AP

    IHC staining of human tonsillitis using 25278-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 25278-1-AP (PRTN3 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human tonsillitis using 25278-1-AP

    IHC staining of human tonsillitis using 25278-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 25278-1-AP (PRTN3 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inhuman placenta tissue
Positive IHC detected inhuman tonsillitis tissue, human colon cancer tissue, human spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:300-1:1200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 25278-1-AP is PRTN3 Fusion Protein expressed in E. coli.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB, IHC
Tested Reactivity human
Cited Reactivityhuman, mouse
Immunogen PRTN3 fusion protein Ag16765 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name proteinase 3
Synonyms ACPA, AGP7, C ANCA, C ANCA antigen, Leukocyte proteinase 3, MBN, MBT, Myeloblastin, Neutrophil proteinase 4, P29, PR 3, PR3, proteinase 3, PRTN3, Wegener autoantigen
Calculated Molecular Weight 256 aa, 28 kDa
Observed Molecular Weight 28 kDa
GenBank Accession NumberBC096183
Gene Symbol PRTN3
Gene ID (NCBI) 5657
RRIDAB_2880003
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP24158
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PRTN3 antibody 25278-1-APDownload protocol
IHC protocol for PRTN3 antibody 25278-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIHC

Biomedicines

Comparative Proteomic Analysis of tPVAT during Ang II Infusion.

Authors - Xiuying Liang
View Article
humanWB

Front Cell Neurosci

Meningitic Escherichia coli-Induced Interleukin-17A Facilitates Blood-Brain Barrier Disruption via Inhibiting Proteinase 3/Protease-Activated Receptor 2 Axis.

Authors - Bojie Xu
View Article
humanWB

Toxicol In Vitro

Activation of inflammasomes by tyrosine kinase inhibitors of vascular endothelial growth factor receptor: Implications for VEGFR TKIs-induced immune related adverse events.

Authors - Hideki Imano
View Article