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Conjugate
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验证数据展示

  • WB analysis of human placenta using 25278-1-AP

    WB analysis of human placenta using 25278-1-AP

    human placenta tissue were subjected to SDS PAGE followed by western blot with 25278-1-AP (PRTN3 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human tonsillitis using 25278-1-AP

    IHC staining of human tonsillitis using 25278-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 25278-1-AP (PRTN3 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human tonsillitis using 25278-1-AP

    IHC staining of human tonsillitis using 25278-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 25278-1-AP (PRTN3 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

PR3/PRTN3 Polyclonal antibody

PR3/PRTN3 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 25278-1-AP

产品说明书

引用文献(3)

宿主/亚型

Rabbit / IgG

种属反应性

human and More (1)

应用

WB, IHC, ELISA

别名

PRTN3, PR3, NP-4, Myeloblastin, MBN

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inhuman placenta tissue
Positive IHC detected inhuman tonsillitis tissue, human colon cancer tissue, human spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:300-1:1200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25278-1-AP targets PR3/PRTN3 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB, IHC
经测试反应性 human
文献引用反应性human, mouse
免疫原 PR3/PRTN3 fusion protein Ag16765 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 proteinase 3
别名 PRTN3, PR3, NP-4, Myeloblastin, MBN
计算分子量 256 aa, 28 kDa
观测分子量 28 kDa
GenBank蛋白编号BC096183
基因名称 PRTN3
Gene ID (NCBI) 5657
RRIDAB_2880003
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP24158
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PR3/PRTN3 antibody 25278-1-APDownload protocol
IHC protocol for PR3/PRTN3 antibody 25278-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIHC

Biomedicines

Comparative Proteomic Analysis of tPVAT during Ang II Infusion.

Authors - Xiuying Liang
View Article
humanWB

Front Cell Neurosci

Meningitic Escherichia coli-Induced Interleukin-17A Facilitates Blood-Brain Barrier Disruption via Inhibiting Proteinase 3/Protease-Activated Receptor 2 Axis.

Authors - Bojie Xu
View Article
humanWB

Toxicol In Vitro

Activation of inflammasomes by tyrosine kinase inhibitors of vascular endothelial growth factor receptor: Implications for VEGFR TKIs-induced immune related adverse events.

Authors - Hideki Imano
View Article