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PRTN3 Polyclonal antibody

PRTN3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 27153-1-AP

Print datasheet

Synonyms

PR3, NP-4, Myeloblastin, MBN, EC:3.4.21.76

验证数据展示

  • WB analysis of human spleen using 27153-1-AP

    WB analysis of human spleen using 27153-1-AP

    human spleen tissue were subjected to SDS PAGE followed by western blot with 27153-1-AP (PRTN3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human spleen using 27153-1-AP

    IHC staining of human spleen using 27153-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 27153-1-AP (PRTN3 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human spleen using 27153-1-AP

    IHC staining of human spleen using 27153-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 27153-1-AP (PRTN3 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human tonsillitis using 27153-1-AP

    IHC staining of human tonsillitis using 27153-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 27153-1-AP (PRTN3 antibody) at dilution of 1:1200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human tonsillitis using 27153-1-AP

    IHC staining of human tonsillitis using 27153-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 27153-1-AP (PRTN3 antibody) at dilution of 1:1200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inhuman spleen tissue, human placenta tissue
Positive IHC detected inhuman spleen tissue, human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27153-1-AP targets PRTN3 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human
Immunogen PRTN3 fusion protein Ag25915 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name proteinase 3
Synonyms PR3, NP-4, Myeloblastin, MBN, EC:3.4.21.76
Calculated Molecular Weight 256 aa, 28 kDa
Observed Molecular Weight 28 kDa
GenBank Accession NumberBC096183
Gene Symbol PRTN3
Gene ID (NCBI) 5657
RRIDAB_2880777
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP24158
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PRTN3 antibody 27153-1-APDownload protocol
IHC protocol for PRTN3 antibody 27153-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols