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PRUNE2 Polyclonal antibody
PRUNE2 Polyclonal Antibody for IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (1)
Applications
IHC, ELISA and More (3)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
11458-1-AP targets PRUNE2 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human samples.
| Tested Applications | IHC, ELISA Application Description |
| Cited Applications | WB, IP, IF, IHC |
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Immunogen | PRUNE2 fusion protein Ag2025 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | prune homolog 2 (Drosophila) |
| Synonyms | A214N16.3, BMCC1, BNIPXL, C9orf65, KIAA0367, Protein prune homolog 2, prune homolog 2 (Drosophila), PRUNE2, RP11 58J3.2 |
| Calculated Molecular Weight | 341 kDa |
| Observed Molecular Weight | 140~150 kDa |
| GenBank Accession Number | BC019095 |
| Gene Symbol | PRUNE2 |
| Gene ID (NCBI) | 158471 |
| RRID | AB_2172439 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8WUY3 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PRUNE2 gene, also named BMCC1, encodes protein prune homolog 2. PRUNE2 gene are overlapping with PCA3, one of the most prostate cancer specific markers. PRUNE2 may play an important role in regulating differentiation, survival and aggressiveness of the tumor cells. Besides its high level of expression seen in the nervous system (brain, cerebellum and spinal cord), it is also expressed at high levels in noneuroblastoma, rhabdomyosarcoma, melanoma and some osteosarcoma cell lines, whereas at only low levels in cancer cell lines of liver, breast, thyroid and colon. The Calculated molecular weight of this protein is 340 kDa. Our antibody specifically recognises the 140-150 kDa band which is an isoform or cleaved fragment of PRUNE2 (PMID:21234814).
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for PRUNE2 antibody 11458-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Proc Natl Acad Sci U S A PRUNE2 is a human prostate cancer suppressor regulated by the intronic long noncoding RNA PCA3.
| ||
Cell Death Dis BMCC1, which is an interacting partner of BCL2, attenuates AKT activity, accompanied by apoptosis. | ||
Exp Ther Med PRUNE2 inhibits progression of colorectal cancer in vitro and in vivo.
| ||
Technol Cancer Res Treat FOXF2 Regulates PRUNE2 Transcription in the Pathogenesis of Colorectal Cancer |