验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, HEK-293 cells, MCF-7 cells, LNCaP cells, mouse brain tissue, mouse heart tissue, rat brain tissue |
| Positive IHC detected in | human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
10664-1-AP targets PRDX3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | PRDX3 fusion protein Ag1062 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | peroxiredoxin 3 |
| 别名 | peroxiredoxin 3, MER5, EC:1.11.1.24, AOP-1, AOP1 |
| 计算分子量 | 27 kDa |
| 观测分子量 | 26-28 kDa |
| GenBank蛋白编号 | BC007062 |
| 基因名称 | PRDX3 |
| Gene ID (NCBI) | 10935 |
| RRID | AB_2284207 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P30048 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PRDX3(Peroxiredoxin 3), also named as AOP1, HBC189 and MER5, belongs to the ahpC/TSA family. It is involved in redox regulation of the cell and protects radical-sensitive enzymes from oxidative damage by a radical-generating system. PRDX3 is required for MYC-mediated proliferation, transformation, and apoptosis after glucose withdrawal and essential for maintaining mitochondrial mass and membrane potential in transformed rat and human cells (PMID:12011429). PRDX3 acts synergistically with MAP3K13 to regulate the activation of NF-kappa-B in the cytosol.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PRDX3 antibody 10664-1-AP | Download protocol |
| IHC protocol for PRDX3 antibody 10664-1-AP | Download protocol |
| IF protocol for PRDX3 antibody 10664-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun Inhibited peroxidase activity of peroxiredoxin 1 by palmitic acid exacerbates nonalcoholic steatohepatitis in male mice | ||
Redox Biol Icariin alleviates uveitis by targeting peroxiredoxin 3 to modulate retinal microglia M1/M2 phenotypic polarization.
| ||
Redox Biol LRPPRC regulates redox homeostasis via the circANKHD1/FOXM1 axis to enhance bladder urothelial carcinoma tumorigenesis. | ||
Oxid Med Cell Longev Paeoniflorin Upregulates Mitochondrial Thioredoxin of Schwann Cells to Improve Diabetic Peripheral Neuropathy Indicated by 4D Label-Free Quantitative Proteomics. | ||
Mol Cell Proteomics Proteomics characterization of the cytotoxicity mechanism of ganoderic acid D and computer-automated estimation of the possible drug target network. |
