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验证数据展示

  • IF Staining of HepG2 using 66810-1-Ig

    IF Staining of HepG2 using 66810-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using PRDX3 antibody (66810-1-Ig, Clone: 1F7F7 ) at dilution of 1:1700 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ).

  • WB analysis using 66810-1-Ig

    WB analysis using 66810-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66810-1-Ig (PRX3 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 66810-1-Ig

    WB analysis of HEK-293 using 66810-1-Ig

    WB result of PRDX3 antibody (66810-1-Ig; 1:80000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PRDX3 transfected HEK-293 cells.

  • IHC staining of human liver cancer using 66810-1-Ig

    IHC staining of human liver cancer using 66810-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66810-1-Ig (PRX3 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PRDX3 antibody included
  • IHC staining of human liver cancer using 66810-1-Ig

    IHC staining of human liver cancer using 66810-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66810-1-Ig (PRX3 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PRDX3 antibody included
  • Featured Product
  • KD/KO验证

PRDX3 Monoclonal antibody

PRDX3 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 66810-1-Ig

产品说明书

CloneNo. 1F7F7
引用文献(2)

宿主/亚型

Mouse / IgG2a

种属反应性

human and More (2)

应用

WB, IHC, IF/ICC, ELISA

别名

MER5, EC:1.11.1.24, AOP-1, AOP1, Antioxidant protein 1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, MCF-7 cells, Jurkat cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy PRDX3 Ready-To-Use IHC Kit. PRDX3 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:20000-1:100000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:850-1:3400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

66810-1-Ig targets PRDX3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB
经测试反应性 human
文献引用反应性rat, pig
免疫原 PRDX3 fusion protein Ag1062 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 peroxiredoxin 3
别名 MER5, EC:1.11.1.24, AOP-1, AOP1, Antioxidant protein 1
计算分子量 27 kDa
观测分子量 28 kDa
GenBank蛋白编号BC007062
基因名称 PRDX3
Gene ID (NCBI) 10935
RRIDAB_2882153
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP30048
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PRDX3 antibody 66810-1-IgDownload protocol
IHC protocol for PRDX3 antibody 66810-1-IgDownload protocol
IF protocol for PRDX3 antibody 66810-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
pigWB

Free Radic Biol Med

Resveratrol and its derivative pterostilbene attenuate oxidative stress-induced intestinal injury by improving mitochondrial redox homeostasis and function via SIRT1 signaling.

Authors - Yanan Chen
View Article
ratWB

Ecotoxicol Environ Saf

Mechanism of KAT2A regulation of H3K36ac in manganese-induced oxidative damage to mitochondria in the nervous system and intervention by curcumin

Authors - Yan Liu
View Article