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PRDX3 Monoclonal antibody

PRDX3 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human and More (2)

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1F7F7

Cat no : 66810-1-Ig

Print datasheet

Synonyms

Antioxidant protein 1, AOP 1, AOP1, HBC189, MER5, peroxiredoxin 3, Peroxiredoxin III, PRDX3, PRO1748, Protein MER5 homolog, Prx III, PRX3, SP 22

验证数据展示

  • WB analysis using 66810-1-Ig

    WB analysis using 66810-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66810-1-Ig (PRX3 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 66810-1-Ig

    WB analysis of HEK-293 using 66810-1-Ig

    WB result of PRDX3 antibody (66810-1-Ig; 1:80000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PRDX3 transfected HEK-293 cells.

经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, MCF-7 cells, Jurkat cells
Planning an IHC experiment? We recommend our IHCeasy PRDX3 Ready-To-Use IHC Kit. PRDX3 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:20000-1:100000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

66810-1-Ig targets PRDX3 in WB, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity Human
Cited Reactivityrat, pig
Immunogen PRDX3 fusion protein Ag1062 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name peroxiredoxin 3
Synonyms Antioxidant protein 1, AOP 1, AOP1, HBC189, MER5, peroxiredoxin 3, Peroxiredoxin III, PRDX3, PRO1748, Protein MER5 homolog, Prx III, PRX3, SP 22
Calculated Molecular Weight 27 kDa
Observed Molecular Weight 28 kDa
GenBank Accession NumberBC007062
Gene Symbol PRDX3
Gene ID (NCBI) 10935
RRIDAB_2882153
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP30048
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PRDX3 antibody 66810-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
pigWB

Free Radic Biol Med

Resveratrol and its derivative pterostilbene attenuate oxidative stress-induced intestinal injury by improving mitochondrial redox homeostasis and function via SIRT1 signaling.

Authors - Yanan Chen
View Article
ratWB

Ecotoxicol Environ Saf

Mechanism of KAT2A regulation of H3K36ac in manganese-induced oxidative damage to mitochondria in the nervous system and intervention by curcumin

Authors - Yan Liu
View Article