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验证数据展示

  • IHC staining of human liver cancer using 67599-1-Ig (same clone as 67599-1-PBS)

    IHC staining of human liver cancer using 67599-1-Ig (same clone as 67599-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67599-1-Ig (PRX5 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67599-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PRDX5 antibody included
  • IHC staining of human liver cancer using 67599-1-Ig (same clone as 67599-1-PBS)

    IHC staining of human liver cancer using 67599-1-Ig (same clone as 67599-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67599-1-Ig (PRX5 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67599-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PRDX5 antibody included
  • IF Staining of HeLa using 67599-1-Ig (same clone as 67599-1-PBS)

    IF Staining of HeLa using 67599-1-Ig (same clone as 67599-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PRX5 antibody (67599-1-Ig, Clone: 1B8C5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67599-1-PBS in a different storage buffer formulation.

  • WB analysis using 67599-1-Ig (same clone as 67599-1-PBS)

    WB analysis using 67599-1-Ig (same clone as 67599-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67599-1-Ig (PRX5 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67599-1-PBS in a different storage buffer formulation.

  • WB analysis using 67599-1-Ig (same clone as 67599-1-PBS)

    WB analysis using 67599-1-Ig (same clone as 67599-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67599-1-Ig (PRX5 antibody) at dilution of 1:5200 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67599-1-PBS in a different storage buffer formulation.

  • WB analysis of HepG2 using 67599-1-Ig (same clone as 67599-1-PBS)

    WB analysis of HepG2 using 67599-1-Ig (same clone as 67599-1-PBS)

    WB result of PRX5 antibody (67599-1-Ig; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PRX5 transfected HepG2 cells. This data was developed using the same antibody clone with 67599-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

PRX5 Monoclonal antibody, PBS Only

PRX5 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 67599-1-PBS

产品说明书

CloneNo. 1B8C5

宿主/亚型

Mouse / IgG1

种属反应性

Human, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

ACR1, Alu corepressor 1, Antioxidant enzyme B166, AOEB166, AOPP, B166, Liver tissue 2D page spot 71B, peroxiredoxin 5, Peroxiredoxin 5, mitochondrial, Peroxiredoxin V, Peroxisomal antioxidant enzyme, PLP, PMP20, PRDX5, PRDX6, Prx V, Prx5, PRXV, SBBI10, Thioredoxin peroxidase PMP20, Thioredoxin reductase, TPx type VI

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy PRDX5 Ready-To-Use IHC Kit. PRDX5 primary antibody included.

产品信息

67599-1-PBS targets PRX5 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 Human, rat
免疫原 PRX5 fusion protein Ag12262 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 peroxiredoxin 5
别名 ACR1, Alu corepressor 1, Antioxidant enzyme B166, AOEB166, AOPP, B166, Liver tissue 2D page spot 71B, peroxiredoxin 5, Peroxiredoxin 5, mitochondrial, Peroxiredoxin V, Peroxisomal antioxidant enzyme, PLP, PMP20, PRDX5, PRDX6, Prx V, Prx5, PRXV, SBBI10, Thioredoxin peroxidase PMP20, Thioredoxin reductase, TPx type VI
计算分子量 214 aa, 22 kDa
观测分子量 17 kDa
GenBank蛋白编号BC110983
基因名称 PRX5
Gene ID (NCBI) 25824
RRIDAB_2882805
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP30044
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Peroxiredoxin 5 (Prx5) is a member of a family consisting of six antioxidant enzymes. Prx5 is ubiquitously expressed in various tissues including mitochondria and peroxisomes, implying that Prx5 functions as a regulator of the cellular oxidation state. Prx5 plays a critical role in protecting cells from oxidative stress by inhibiting the accumulation of reactive oxygen species and cell death. Prx5 has an intensive ROS scavenging activity because it is located in the cytosol and mitochondria. Prx5 expression was upregulated during adipogenesis and Prx5 overexpression suppressed adipogenesis by regulating cytosolic and mitochondrial ROS generation. (PMID: 29777756, PMID: 31505325, PMID: 22020876)