验证数据展示
经过测试的应用
Positive WB detected in | HeLa cells, HepG2 cells |
Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | PC-3 cells |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:1000-1:4000 |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 2 publications below |
CoIP | See 1 publications below |
产品信息
18398-1-AP targets PSAP in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human samples.
经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
文献引用应用 | WB, CoIP |
经测试反应性 | human |
文献引用反应性 | human |
免疫原 | PSAP fusion protein Ag13265 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | prosaposin |
别名 | Dispersin, CSAct, Component C, Co beta glucosidase, Cerebroside sulfate activator |
计算分子量 | 58 kDa |
观测分子量 | 60-70 kDa |
GenBank蛋白编号 | BC001503 |
基因名称 | PSAP |
Gene ID (NCBI) | 5660 |
RRID | AB_10598315 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | P07602 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
proteins' (SAPs) that assist in the lysosomal hydrolysis of sphingolipids. After proteolytic processing of the presaposin protein, these 4 released polypeptides are functional activators. Saposin A was encoded by residues 60 to 143 of PSAP, saposin B by 195 to 275, saposin C by 311 to 390, and saposin D by 405 to 487. There are four 12-14 kDa heatstable glycoproteins. This antibody should recognize saposin C.
实验方案
Product Specific Protocols | |
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WB protocol for PSAP antibody 18398-1-AP | Download protocol |
IHC protocol for PSAP antibody 18398-1-AP | Download protocol |
IF protocol for PSAP antibody 18398-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Hum Mol Genet Lysosomal ceramides regulate Cathepsin B-mediated processing of saposin C and glucocerebrosidase activity. | ||
JCI Insight Inhibition of cysteine protease cathepsin Lincreases the level and activity of lysosomal glucocerebrosidase |