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验证数据展示

  • WB analysis using 18423-1-AP

    WB analysis using 18423-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 18423-1-AP (PSAP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 18423-1-AP

    IHC staining of human liver cancer using 18423-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer using 18423-1-AP (PSAP antibody) at dilution of 1:50 (under 10x lens).

    Ready-to-use IHC kits available with PSAP antibody included
  • IHC staining of human liver cancer using 18423-1-AP

    IHC staining of human liver cancer using 18423-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer using 18423-1-AP (PSAP antibody) at dilution of 1:50 (under 40x lens).

    Ready-to-use IHC kits available with PSAP antibody included
  • WB analysis of HepG2 using 18423-1-AP

    WB analysis of HepG2 using 18423-1-AP

    WB result of PSAP antibody (18423-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PSAP transfected HepG2 cells.

  • Featured Product
  • KD/KO验证

PSAP Polyclonal antibody

PSAP Polyclonal Antibody for WB, IHC, ELISA
Cat No. 18423-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

A1 activator, Cerebroside sulfate activator, Co beta glucosidase, Component C, CSAct, Dispersin, GLBA, Glucosylceramidase activator, Proactivator polypeptide, prosaposin, Protein C, PSAP, SAP 1, SAP 2 Saposin D, SAP1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293T cells, HeLa cells, HepG2 cells
Positive IHC detected inhuman liver cancer tissue, human prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy PSAP Ready-To-Use IHC Kit. PSAP primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

18423-1-AP targets PSAP in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原 PSAP fusion protein Ag13266 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 prosaposin
别名 A1 activator, Cerebroside sulfate activator, Co beta glucosidase, Component C, CSAct, Dispersin, GLBA, Glucosylceramidase activator, Proactivator polypeptide, prosaposin, Protein C, PSAP, SAP 1, SAP 2 Saposin D, SAP1
计算分子量 58 kDa
观测分子量 60-70 kDa
GenBank蛋白编号BC001503
基因名称 PSAP
Gene ID (NCBI) 5660
RRIDAB_10643525
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP07602
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

proteins' (SAPs) that assist in the lysosomal hydrolysis of sphingolipids. After proteolytic processing of the presaposin protein, these 4 released polypeptides are functional activators. Saposin A was encoded by residues 60 to 143 of PSAP, saposin B by 195 to 275, saposin C by 311 to 390, and saposin D by 405 to 487. There are four 12-14 kDa heatstable glycoproteins. This antibody should recognize saposin D.

实验方案

Product Specific Protocols
WB protocol for PSAP antibody 18423-1-APDownload protocol
IHC protocol for PSAP antibody 18423-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols