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PSAP Polyclonal antibody

PSAP Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 18423-1-AP

Print datasheet

Synonyms

A1 activator, Cerebroside sulfate activator, Co beta glucosidase, Component C, CSAct, Dispersin, GLBA, Glucosylceramidase activator, Proactivator polypeptide, prosaposin, Protein C, PSAP, SAP 1, SAP 2 Saposin D, SAP1

验证数据展示

  • WB analysis using 18423-1-AP

    WB analysis using 18423-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 18423-1-AP (PSAP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 18423-1-AP

    IHC staining of human liver cancer using 18423-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer using 18423-1-AP (PSAP antibody) at dilution of 1:50 (under 10x lens).

    Ready-to-use IHC kits available with PSAP antibody included
  • IHC staining of human liver cancer using 18423-1-AP

    IHC staining of human liver cancer using 18423-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer using 18423-1-AP (PSAP antibody) at dilution of 1:50 (under 40x lens).

    Ready-to-use IHC kits available with PSAP antibody included
  • WB analysis of HepG2 using 18423-1-AP

    WB analysis of HepG2 using 18423-1-AP

    WB result of PSAP antibody (18423-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PSAP transfected HepG2 cells.

经过测试的应用

Positive WB detected inHEK-293T cells, HeLa cells, HepG2 cells
Positive IHC detected inhuman liver cancer tissue, human prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy PSAP Ready-To-Use IHC Kit. PSAP primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

18423-1-AP targets PSAP in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human
Immunogen PSAP fusion protein Ag13266 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name prosaposin
Synonyms A1 activator, Cerebroside sulfate activator, Co beta glucosidase, Component C, CSAct, Dispersin, GLBA, Glucosylceramidase activator, Proactivator polypeptide, prosaposin, Protein C, PSAP, SAP 1, SAP 2 Saposin D, SAP1
Calculated Molecular Weight 58 kDa
Observed Molecular Weight 60-70 kDa
GenBank Accession NumberBC001503
Gene Symbol PSAP
Gene ID (NCBI) 5660
RRIDAB_10643525
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP07602
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

proteins' (SAPs) that assist in the lysosomal hydrolysis of sphingolipids. After proteolytic processing of the presaposin protein, these 4 released polypeptides are functional activators. Saposin A was encoded by residues 60 to 143 of PSAP, saposin B by 195 to 275, saposin C by 311 to 390, and saposin D by 405 to 487. There are four 12-14 kDa heatstable glycoproteins. This antibody should recognize saposin D.

实验方案

Product Specific Protocols
WB protocol for PSAP antibody 18423-1-APDownload protocol
IHC protocol for PSAP antibody 18423-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols