验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells, K-562 cells, NIH/3T3 cells, pig brain tissue |
| Positive IHC detected in | human ovarian cancer Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:300-1:1200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
81479-1-RR targets PSAT1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, pig samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 经测试反应性 | human, mouse, pig |
| 免疫原 |
CatNo: Ag0776 Product name: Recombinant human PSAT1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-370 aa of BC004863 Sequence: MDAPRQVVNFGPGPAKLPHSVLLEIQKELLDYKGVGISVLEMSHRSSDFAKIINNTENLVRELLAVPDNYKVIFLQGGGCGQFSAVPLNLIGLKAGRCADYVVTGAWSAKAAEEAKKFGTINIVHPKLGSYTKIPDPSTWNLNPDASYVYYCANETVHGVEFDFIPDVKGAVLVCDMSSNFLSKPVDVSKFGVIFAGAQKNVGSAGVTVVIVRDDLLGFALRECPSVLEYKVQAGNSSLYNTPPCFSIYVMGLVLEWIKNNGGAAAMEKLSSIKSQTIYEIIDNSQGFYVCPVEPQNRSKMNIPFRIGNAKGDDALEKRFLDKALELNMLSLKGHRSVGGIRASLYNAVTIEDVQKLAAFMKKFLEMHQL 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | phosphoserine aminotransferase 1 |
| 别名 | 1O22, EC:2.6.1.52, EPIP, Phosphoserine aminotransferase, PSA |
| 计算分子量 | 40 kDa |
| 观测分子量 | 37-40 kDa |
| GenBank蛋白编号 | BC004863 |
| 基因名称 | PSAT1 |
| Gene ID (NCBI) | 29968 |
| RRID | AB_2935554 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q9Y617 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PSAT1, also named as PSA and PSAT, belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family and SerC subfamily. It catalyzes the reversible conversion of 3-phosphohydroxypyruvate to phosphoserine and of 3-hydroxy-2-oxo-4-phosphonooxybutanoate to phosphohydroxythreonine. PSAT1 represents a new interesting target for CRC therapy. It may be implicated in altered serine metabolism and schizophrenia spectrum conditions. (PMID: 18221502, 20955740)
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for PSAT1 antibody 81479-1-RR | Download protocol |
| IHC protocol for PSAT1 antibody 81479-1-RR | Download protocol |
| WB protocol for PSAT1 antibody 81479-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
