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PSAT1 Recombinant antibody

PSAT1 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse, Pig

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1O22

Cat no : 81479-1-RR

Print datasheet

Synonyms

EPIP, Phosphoserine aminotransferase, PSA, PSAT, PSAT1

验证数据展示

  • WB analysis using 81479-1-RR

    WB analysis using 81479-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 81479-1-RR (PSAT1 antibody) at dilution of 1:12000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 81479-1-RR

    IF Staining of HepG2 using 81479-1-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using PSAT1 antibody (81479-1-RR, Clone: 1O22 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

经过测试的应用

Positive WB detected inHepG2 cells, K-562 cells, NIH/3T3 cells, pig brain tissue
Positive IF/ICC detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

81479-1-RR targets PSAT1 in WB, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Pig samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity Human, Mouse, Pig
Immunogen PSAT1 fusion protein Ag0776 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name phosphoserine aminotransferase 1
Synonyms EPIP, Phosphoserine aminotransferase, PSA, PSAT, PSAT1
Calculated Molecular Weight 40 kDa
Observed Molecular Weight 37-40 kDa
GenBank Accession NumberBC004863
Gene Symbol PSAT1
Gene ID (NCBI) 29968
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9Y617
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PSAT1, also named as PSA and PSAT, belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family and SerC subfamily. It catalyzes the reversible conversion of 3-phosphohydroxypyruvate to phosphoserine and of 3-hydroxy-2-oxo-4-phosphonooxybutanoate to phosphohydroxythreonine. PSAT1 represents a new interesting target for CRC therapy. It may be implicated in altered serine metabolism and schizophrenia spectrum conditions. (PMID: 18221502, 20955740)

实验方案

Product Specific Protocols
WB protocol for PSAT1 antibody 81479-1-RRDownload protocol
IF protocol for PSAT1 antibody 81479-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols