验证数据展示
经过测试的应用
| Positive IHC detected in | human gliomas tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | mouse brain tissue, HEK-293 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:50-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IF | See 1 publications below |
产品信息
20434-1-AP targets PSEN1 in IF, IHC, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | IF, IHC, ELISA Application Description |
| 文献引用应用 | IF |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | mouse |
| 免疫原 | PSEN1 fusion protein Ag14135 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | presenilin 1 |
| 别名 | AD3, FAD, presenilin 1, Protein S182, PS 1, PS1, PS1 CTF12, PSEN1, PSNL1, S182 |
| 计算分子量 | 467 aa, 53 kDa |
| 观测分子量 | 18 kDa |
| GenBank蛋白编号 | BC011729 |
| 基因名称 | PSEN1 |
| Gene ID (NCBI) | 5663 |
| RRID | AB_2878685 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P49768 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PSEN1(presenilin-1) is also named as AD3, PS1, PSNL1 and belongs to the peptidase A22A family. It directly influences Netrin/DCC signaling with cell type precision and spatiotemporal specificity, and is a key neural circuit builder that gates the spatiotemporal pattern of guidance signaling, thereby ensuring neural projections occur with high fidelity(PMID:21215373). The 46-kDa PSEN1 holoprotein is cleaved into a ~30-kDa N-terminal fragment (NTF) and a ~20-kDa C-terminal fragment (CTF) by the 26S proteasome(PMID:10527805). It has 7 isoforms produced by alternative splicing. This antibody can recognize all the isoforms except isoform 4 (21 kDa).
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for PSEN1 antibody 20434-1-AP | Download protocol |
| IF protocol for PSEN1 antibody 20434-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
