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验证数据展示

  • WB analysis using 16144-1-AP

    WB analysis using 16144-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16144-1-AP (PSENEN antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 16144-1-AP

    IHC staining of mouse brain using 16144-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16144-1-AP (PSENEN Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 16144-1-AP

    IHC staining of mouse brain using 16144-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16144-1-AP (PSENEN Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

PSENEN Polyclonal antibody

PSENEN Polyclonal Antibody for WB, IHC, ELISA
Cat No. 16144-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

Presenilin enhancer protein 2, PEN2, PEN 2, Gamma-secretase subunit PEN-2, Gamma secretase subunit PEN 2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA549 cells, Neuro-2a cells, mouse lung tissue, rat spleen tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16144-1-AP targets PSENEN in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 PSENEN fusion protein Ag9243 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 presenilin enhancer 2 homolog (C. elegans)
别名 Presenilin enhancer protein 2, PEN2, PEN 2, Gamma-secretase subunit PEN-2, Gamma secretase subunit PEN 2
计算分子量 101 aa, 12 kDa
观测分子量12 kDa
GenBank蛋白编号BC009575
基因名称 PSENEN
Gene ID (NCBI) 55851
RRIDAB_3669231
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NZ42
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PSENEN, also named PEN2, is a component of the gamma-secretase complex, which also includes presenilin (PSEN1) and nicastrin (NCSTN). The gamma-secretase complex is required for the intramembrane proteolysis of a number of membrane proteins, including the amyloid-beta precursor protein (APP) and Notch. PSENEN is required for Notch pathway signaling, and for the activity and accumulation of gamma-secretase. Mutations resulting in haploinsufficiency for this gene cause familial acne inversa-2 (ACNINV2).

实验方案

Product Specific Protocols
WB protocol for PSENEN antibody 16144-1-APDownload protocol
IHC protocol for PSENEN antibody 16144-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols