验证数据展示
经过测试的应用
| Positive WB detected in | A549 cells, SH-SY5Y cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
83818-1-RR targets PSENEN in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 | PSENEN fusion protein Ag9243 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | presenilin enhancer 2 homolog (C. elegans) |
| 别名 | Presenilin enhancer protein 2, PEN2, PEN 2, Gamma-secretase subunit PEN-2, Gamma secretase subunit PEN 2 |
| 计算分子量 | 101 aa, 12 kDa |
| 观测分子量 | 12 kDa |
| GenBank蛋白编号 | BC009575 |
| 基因名称 | PSENEN |
| Gene ID (NCBI) | 55851 |
| RRID | AB_3671404 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purfication |
| UNIPROT ID | Q9NZ42 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PSENEN, also named PEN2, is a component of the gamma-secretase complex, which also includes presenilin (PSEN1) and nicastrin (NCSTN). The gamma-secretase complex is required for the intramembrane proteolysis of a number of membrane proteins, including the amyloid-beta precursor protein (APP) and Notch. PSENEN is required for Notch pathway signaling, and for the activity and accumulation of gamma-secretase. Mutations resulting in haploinsufficiency for this gene cause familial acne inversa-2 (ACNINV2).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PSENEN antibody 83818-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |