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PSG1 Polyclonal antibody

PSG1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 11809-1-AP

Print datasheet

Synonyms

FL NCA 1/2, Fetal liver non-specific cross-reactive antigen 1/2, DHFRP2, CD66f, CD66 antigen-like family member F

验证数据展示

  • WB analysis of human kidney using 11809-1-AP

    WB analysis of human kidney using 11809-1-AP

    human kidney tissue were subjected to SDS PAGE followed by western blot with 11809-1-AP (PSG1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human placenta using 11809-1-AP

    IHC staining of human placenta using 11809-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 11809-1-AP (PSG1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human placenta using 11809-1-AP

    IHC staining of human placenta using 11809-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 11809-1-AP (PSG1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of MCF-7 using 11809-1-AP

    IF Staining of MCF-7 using 11809-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using PSG1 antibody (11809-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

经过测试的应用

Positive WB detected inhuman kidney tissue, MCF-7 cells
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IFSee 1 publications below

产品信息

11809-1-AP targets PSG1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsIF
Tested Reactivity human
Cited Reactivityhuman
Immunogen PSG1 fusion protein Ag2468 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name pregnancy specific beta-1-glycoprotein 1
Synonyms FL NCA 1/2, Fetal liver non-specific cross-reactive antigen 1/2, DHFRP2, CD66f, CD66 antigen-like family member F
Calculated Molecular Weight 426 aa, 48 kDa
Observed Molecular Weight 72 kDa, 36 kDa
GenBank Accession NumberBC022338
Gene Symbol PSG1
Gene ID (NCBI) 5669
RRIDAB_2171045
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP11464
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PSG1 antibody 11809-1-APDownload protocol
IHC protocol for PSG1 antibody 11809-1-APDownload protocol
IF protocol for PSG1 antibody 11809-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

Fertil Steril

Trophoblast retrieval and isolation from the cervix (TRIC) for noninvasive prenatal screening at 5 to 20 weeks of gestation.

Authors - Jay M Bolnick
View Article