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PSIP1 Polyclonal antibody

PSIP1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 25504-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA and More (2)

PC4 and SFRS1-interacting protein, Lens epithelium-derived growth factor, LEDGF, DFS70, CLL-associated antigen KW-7

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, Jurkat cells, NIH/3T3 cells
Positive IHC detected inmouse brain tissue, mouse spleen tissue, rat brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy PSIP1 Ready-To-Use IHC Kit. PSIP1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:16000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25504-1-AP targets PSIP1 in WB, IHC, IF/ICC, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB, IF, RIP
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原 PSIP1 fusion protein Ag21886 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 PC4 and SFRS1 interacting protein 1
别名 PC4 and SFRS1-interacting protein, Lens epithelium-derived growth factor, LEDGF, DFS70, CLL-associated antigen KW-7
计算分子量 530 aa, 60 kDa
观测分子量75 kDa
GenBank蛋白编号BC064135
基因名称 PSIP1
Gene ID (NCBI) 11168
RRIDAB_2861312
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO75475
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PSIP1 is a chromatin associated protein that is shown to act as a transcriptional coactivator as well as an RNA-binding protein. PSIP1 encodes two protein isoforms with molecular masses of 52 and 75 kDa. p52 and p75 were identified as interacting with transcription factor PC4 and shown to act as transcriptional coactivators.

实验方案

Product Specific Protocols
WB protocol for PSIP1 antibody 25504-1-APDownload protocol
IHC protocol for PSIP1 antibody 25504-1-APDownload protocol
IF protocol for PSIP1 antibody 25504-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Sci Adv

The H3K36me2 writer-reader dependency in H3K27M-DIPG.

Authors - Jia-Ray Yu
  • KD Validated
humanWB

Sci Adv

LEDGF and HDGF2 relieve the nucleosome-induced barrier to transcription in differentiated cells.

Authors - Gary LeRoy
  • KO Validated
humanRIP

Elife

The Tudor SND1 protein is an m6A RNA reader essential for replication of Kaposi's sarcoma-associated herpesvirus.

Authors - Belinda Baquero-Perez
humanIF,WB

Front Immunol

Single-cell RNA-seq reveals T cell exhaustion and immune response landscape in osteosarcoma

Authors - Qizhi Fan
humanWB

Cancer Sci

N-acetylgalactosaminyltransferase GALNT6 is a potential therapeutic target of clear cell renal cell carcinoma progression

Authors - Luhaoran Sun
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