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PSKH2 Polyclonal antibody

PSKH2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 18813-1-AP

Print datasheet

Synonyms

Serine/threonine-protein kinase H2, PSK-H2, PSK H2, Protein serine kinase H2, EC:2.7.11.1

验证数据展示

  • WB analysis of HepG2 using 18813-1-AP

    WB analysis of HepG2 using 18813-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 18813-1-AP (PSKH2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 18813-1-AP

    IHC staining of human kidney using 18813-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 18813-1-AP (PSKH2 antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human kidney using 18813-1-AP

    IHC staining of human kidney using 18813-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 18813-1-AP (PSKH2 antibody) at dilution of 1:100 (under 40x lens).

  • IP experiment of HepG2 using 18813-1-AP

    IP experiment of HepG2 using 18813-1-AP

    IP result of anti-PSKH2 (IP:18813-1-AP, 4ug; Detection:18813-1-AP 1:500) with HepG2 cells lysate 3400ug.

  • IF Staining of HepG2 using 18813-1-AP

    IF Staining of HepG2 using 18813-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 18813-1-AP (PSKH2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHepG2 cells, human brain tissue, human heart tissue, human testis tissue
Positive IP detected inHepG2 cells
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

18813-1-AP targets PSKH2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Tested Reactivity human
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name protein serine kinase H2
Synonyms Serine/threonine-protein kinase H2, PSK-H2, PSK H2, Protein serine kinase H2, EC:2.7.11.1
Calculated Molecular Weight 43 kDa
Observed Molecular Weight 35 kDa
GenBank Accession NumberBC126182
Gene Symbol PSKH2
Gene ID (NCBI) 85481
RRIDAB_2171244
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96QS6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PSKH2 belongs to the protein kinase superfamily and CAMK Ser/Thr protein kinase family. PSKH2 catalytic the reaction: ATP + a protein = ADP + a phosphoprotein.

实验方案

Product Specific Protocols
WB protocol for PSKH2 antibody 18813-1-APDownload protocol
IHC protocol for PSKH2 antibody 18813-1-APDownload protocol
IF protocol for PSKH2 antibody 18813-1-APDownload protocol
IP protocol for PSKH2 antibody 18813-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols