验证数据展示
经过测试的应用
Positive WB detected in | COLO 320 cells, HeLa cells, PC-3 cells, mouse kidney tissue |
Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | PC-3 cells |
推荐稀释比
Application | Dilution |
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Western Blot (WB) | WB : 1:1000-1:4000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 1 publications below |
产品信息
11175-1-AP targets PSMA1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | WB, IHC, IF/ICC, ELISA Application Description |
Cited Applications | WB |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | rat |
Immunogen | PSMA1 fusion protein Ag1660 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | proteasome (prosome, macropain) subunit, alpha type, 1 |
Synonyms | PSC2, Proteasome subunit alpha type-1, PROS-30, PROS30, NU |
Calculated Molecular Weight | 30 kDa |
Observed Molecular Weight | 29-33 kDa |
GenBank Accession Number | BC015105 |
Gene Symbol | PSMA1 |
Gene ID (NCBI) | 5682 |
RRID | AB_2171377 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P25786 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PSMA1(Proteasome subunit alpha type-1) is also named as HC2, NU, PROS30, PSC2 and belongs to the peptidase T1A family.The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH.It is induced in breast cancer tissue and up-regulated in liver tumor tissues(PMID:17127214;16317774;17004105).
实验方案
Product Specific Protocols | |
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WB protocol for PSMA1 antibody 11175-1-AP | Download protocol |
IHC protocol for PSMA1 antibody 11175-1-AP | Download protocol |
IF protocol for PSMA1 antibody 11175-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |