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验证数据展示

  • IF Staining of PC-3 using 11175-1-AP

    IF Staining of PC-3 using 11175-1-AP

    Immunofluorescent analysis of PC-3 cells using 11175-1-AP (PSMA1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • WB analysis using 11175-1-AP

    WB analysis using 11175-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11175-1-AP (PSMA1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 11175-1-AP

    IHC staining of human colon cancer using 11175-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11175-1-AP (PSMA1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PSMA1 antibody included

PSMA1 Polyclonal antibody

PSMA1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 11175-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

PSC2, Proteasome subunit alpha type-1, PROS-30, PROS30, NU

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inCOLO 320 cells, HeLa cells, PC-3 cells, mouse kidney tissue
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inPC-3 cells
Planning an IHC experiment? We recommend our IHCeasy PSMA1 Ready-To-Use IHC Kit. PSMA1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

11175-1-AP targets PSMA1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityrat
Immunogen PSMA1 fusion protein Ag1660 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name proteasome (prosome, macropain) subunit, alpha type, 1
Synonyms PSC2, Proteasome subunit alpha type-1, PROS-30, PROS30, NU
Calculated Molecular Weight 30 kDa
Observed Molecular Weight 29-33 kDa
GenBank Accession NumberBC015105
Gene Symbol PSMA1
Gene ID (NCBI) 5682
RRIDAB_2171377
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP25786
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PSMA1(Proteasome subunit alpha type-1) is also named as HC2, NU, PROS30, PSC2 and belongs to the peptidase T1A family.The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH.It is induced in breast cancer tissue and up-regulated in liver tumor tissues(PMID:17127214;16317774;17004105).

实验方案

Product Specific Protocols
WB protocol for PSMA1 antibody 11175-1-APDownload protocol
IHC protocol for PSMA1 antibody 11175-1-APDownload protocol
IF protocol for PSMA1 antibody 11175-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
ratWB

Int J Mol Sci

Impaired 26S Proteasome Assembly Precedes Neuronal Loss in Mutant UBQLN2 Rats.

Authors - Wenjuan Zhang
View Article