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PSMA2 Polyclonal antibody

PSMA2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 14377-1-AP

Print datasheet

Synonyms

PSC3, Proteasome subunit alpha type-2, HC3

验证数据展示

  • IHC staining of human skeletal muscle using 14377-1-AP

    IHC staining of human skeletal muscle using 14377-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 14377-1-AP (PSMA2 antibody) at dilution of 1:200 (under 10x lens).

    Ready-to-use IHC kits available with PSMA2 antibody included
  • IHC staining of human skeletal muscle using 14377-1-AP

    IHC staining of human skeletal muscle using 14377-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 14377-1-AP (PSMA2 antibody) at dilution of 1:200 (under 40x lens).

    Ready-to-use IHC kits available with PSMA2 antibody included
  • WB analysis of NIH/3T3 using 14377-1-AP

    WB analysis of NIH/3T3 using 14377-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 14377-1-AP (PSMA2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 14377-1-AP

    IF Staining of HepG2 using 14377-1-AP

    Immunofluorescent analysis of HepG2 cells using 14377-1-AP (PSMA2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inNIH/3T3 cells, HEK-293 cells, HepG2 cells, mouse kidney tissue, PC-3 cells
Positive IHC detected inhuman skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy PSMA2 Ready-To-Use IHC Kit. PSMA2 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14377-1-AP targets PSMA2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB, IHC
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen PSMA2 fusion protein Ag5753 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name proteasome (prosome, macropain) subunit, alpha type, 2
Synonyms PSC3, Proteasome subunit alpha type-2, HC3
Calculated Molecular Weight 26 kDa
Observed Molecular Weight 26 kDa
GenBank Accession NumberBC047697
Gene Symbol PSMA2
Gene ID (NCBI) 5683
RRIDAB_2878050
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP25787
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PSMA2 (Proteasome subunit alpha type-2) is the essential subunit that contributes to the complete assembly of 20S proteasome complex, involved in the proteolytic degradation of most intracellular proteins. It is required in spermatogenesis and generation of a subset of MHC class I-presented antigenic peptides(PMID: 34711951, PMID: 27342858). Dysregulation of PSMA2 degradation leads to tumorigenesis including cervical cancer (PMID:33684886).

实验方案

Product Specific Protocols
WB protocol for PSMA2 antibody 14377-1-APDownload protocol
IHC protocol for PSMA2 antibody 14377-1-APDownload protocol
IF protocol for PSMA2 antibody 14377-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Acta Biomater

Pushpin-like nanozyme for plasmon-enhanced tumor targeted therapy

Authors - Baofu Ma
View Article
humanIHC

J Ovarian Res

Development and clinical validation of a seven-gene signature based on tumor stem cell-related genes to predict ovarian cancer prognosis

Authors - Guangwei Wang
View Article
humanWB

J Gastroenterol

Corynoline protects chronic pancreatitis via binding to PSMA2 and alleviating pancreatic fibrosis

Authors - Pengyuan Wang
View Article