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PSMA4 Monoclonal antibody

PSMA4 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Pig, Rabbit, Rat, Mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2E6E4

Cat no : 68203-1-Ig

Print datasheet

Synonyms

HC9, HsT17706, Macropain subunit C9, Proteasome component C9, Proteasome subunit L, PSC9, PSMA4

验证数据展示

  • WB analysis using 68203-1-Ig

    WB analysis using 68203-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68203-1-Ig (PSMA4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IHC staining of human pancreas cancer using 68203-1-Ig

    IHC staining of human pancreas cancer using 68203-1-Ig

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 68203-1-Ig (PSMA4 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PSMA4 antibody included
  • IF Staining of HeLa using 68203-1-Ig

    IF Staining of HeLa using 68203-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using PSMA4 antibody (68203-1-Ig, Clone: 2E6E4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

经过测试的应用

Positive WB detected inLNCaP cells, HeLa cells, K-562 cells, Pig heart, Rabbit heart, Rat heart, Mouse heart
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy PSMA4 Ready-To-Use IHC Kit. PSMA4 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68203-1-Ig targets PSMA4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, Pig, Rabbit, Rat, Mouse samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity Human, Pig, Rabbit, Rat, Mouse
Immunogen PSMA4 fusion protein Ag32626 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name proteasome (prosome, macropain) subunit, alpha type, 4
Synonyms HC9, HsT17706, Macropain subunit C9, Proteasome component C9, Proteasome subunit L, PSC9, PSMA4
Calculated Molecular Weight 237 aa, 27 kDa
Observed Molecular Weight29 kDa
GenBank Accession NumberBC056249
Gene Symbol PSMA4
Gene ID (NCBI) 5685
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP25789
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PSMA4 antibody 68203-1-IgDownload protocol
IHC protocol for PSMA4 antibody 68203-1-IgDownload protocol
IF protocol for PSMA4 antibody 68203-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols