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验证数据展示

  • WB analysis using 68203-1-Ig

    WB analysis using 68203-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68203-1-Ig (PSMA4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IHC staining of human pancreas cancer using 68203-1-Ig

    IHC staining of human pancreas cancer using 68203-1-Ig

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 68203-1-Ig (PSMA4 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PSMA4 antibody included
  • IF Staining of HeLa using 68203-1-Ig

    IF Staining of HeLa using 68203-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using PSMA4 antibody (68203-1-Ig, Clone: 2E6E4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

PSMA4 Monoclonal antibody

PSMA4 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 68203-1-Ig

产品说明书

CloneNo. 2E6E4

宿主/亚型

Mouse / IgG2b

种属反应性

Human, Pig, Rabbit, Rat, Mouse

应用

WB, IHC, IF/ICC, ELISA

别名

HC9, HsT17706, Macropain subunit C9, Proteasome component C9, Proteasome subunit L, PSC9, PSMA4

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inLNCaP cells, HeLa cells, K-562 cells, Pig heart, Rabbit heart, Rat heart, Mouse heart
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy PSMA4 Ready-To-Use IHC Kit. PSMA4 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68203-1-Ig targets PSMA4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, Pig, Rabbit, Rat, Mouse samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 Human, Pig, Rabbit, Rat, Mouse
免疫原 PSMA4 fusion protein Ag32626 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 proteasome (prosome, macropain) subunit, alpha type, 4
别名 HC9, HsT17706, Macropain subunit C9, Proteasome component C9, Proteasome subunit L, PSC9, PSMA4
计算分子量 237 aa, 27 kDa
观测分子量29 kDa
GenBank蛋白编号BC056249
基因名称 PSMA4
Gene ID (NCBI) 5685
RRIDAB_2935291
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP25789
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PSMA4 antibody 68203-1-IgDownload protocol
IHC protocol for PSMA4 antibody 68203-1-IgDownload protocol
IF protocol for PSMA4 antibody 68203-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols