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验证数据展示

  • WB analysis using 68123-1-Ig

    WB analysis using 68123-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68123-1-Ig (PSMA8 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of rat lung using 68123-1-Ig

    WB analysis of rat lung using 68123-1-Ig

    rat lung tissue were subjected to SDS PAGE followed by western blot with 68123-1-Ig (PSMA8 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse lung using 68123-1-Ig

    WB analysis of mouse lung using 68123-1-Ig

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 68123-1-Ig (PSMA8 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 68123-1-Ig

    WB analysis of HeLa using 68123-1-Ig

    WB result of PSMA8 antibody (68123-1-Ig; 1:80000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PSMA8 transfected HeLa cells.

  • IHC staining of mouse testis using 68123-1-Ig

    IHC staining of mouse testis using 68123-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 68123-1-Ig (PSMA8 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PSMA8 antibody included
  • IHC staining of mouse testis using 68123-1-Ig

    IHC staining of mouse testis using 68123-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 68123-1-Ig (PSMA8 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PSMA8 antibody included
  • IF Staining of HepG2 using 68123-1-Ig

    IF Staining of HepG2 using 68123-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using PSMA8 antibody (68123-1-Ig, Clone: 2G2G3 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • Featured Product
  • KD/KO验证

PSMA8 Monoclonal antibody

PSMA8 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 68123-1-Ig

产品说明书

CloneNo. 2G2G3

宿主/亚型

Mouse / IgG2a

种属反应性

Human, mouse, rat, pig, rabbit

应用

WB, IHC, IF/ICC, ELISA

别名

PSMA7L, PSMA8

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inHCT 116 cells, rat lung tissue, mouse lung tissue, HeLa cells, HuH-7 cells, NCI-H1299 cells, A549 cells, pig lung tissue, rabbit lung tissue
Positive IHC detected inmouse testis tissue, human colon cancer tissue, human lung cancer tissue, mouse liver tissue, rat liver tissue, rat testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy PSMA8 Ready-To-Use IHC Kit. PSMA8 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68123-1-Ig targets PSMA8 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat, pig, rabbit samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 Human, mouse, rat, pig, rabbit
免疫原 PSMA8 fusion protein Ag5138 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 proteasome (prosome, macropain) subunit, alpha type, 8
别名 PSMA7L, PSMA8
计算分子量 29 kDa
观测分子量28 kDa
GenBank蛋白编号BC042820
基因名称 PSMA8
Gene ID (NCBI) 143471
RRIDAB_2923651
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ8TAA3
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

1

实验方案

Product Specific Protocols
WB protocol for PSMA8 antibody 68123-1-IgDownload protocol
IHC protocol for PSMA8 antibody 68123-1-IgDownload protocol
IF protocol for PSMA8 antibody 68123-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols