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PSMB10 Monoclonal antibody, PBS Only

PSMB10 Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, pig, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1G8C1

Cat no : 66403-1-PBS

Print datasheet

Synonyms

beta2i, LMP10, Low molecular mass protein 10, Macropain subunit MECl 1, MECL1, Proteasome MECl 1, Proteasome subunit beta 2i, PSMB10

验证数据展示

  • WB analysis using 66403-1-Ig (same clone as 66403-1-PBS)

    WB analysis using 66403-1-Ig (same clone as 66403-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66403-1-Ig (PSMB10 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66403-1-PBS in a different storage buffer formulation.

  • WB analysis of Raji using 66403-1-Ig (same clone as 66403-1-PBS)

    WB analysis of Raji using 66403-1-Ig (same clone as 66403-1-PBS)

    Raji cells were subjected to SDS PAGE followed by western blot with 66403-1-Ig (PSMB10 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66403-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 66403-1-Ig (same clone as 66403-1-PBS)

    IHC staining of human liver cancer using 66403-1-Ig (same clone as 66403-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66403-1-Ig (PSMB10 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66403-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PSMB10 antibody included
  • IHC staining of human liver cancer using 66403-1-Ig (same clone as 66403-1-PBS)

    IHC staining of human liver cancer using 66403-1-Ig (same clone as 66403-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66403-1-Ig (PSMB10 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66403-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PSMB10 antibody included
  • WB analysis of rat thymus using 66403-1-Ig (same clone as 66403-1-PBS)

    WB analysis of rat thymus using 66403-1-Ig (same clone as 66403-1-PBS)

    rat thymus tissue were subjected to SDS PAGE followed by western blot with 66403-1-Ig (PSMB10 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66403-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy PSMB10 Ready-To-Use IHC Kit. PSMB10 primary antibody included.

产品信息

66403-1-PBS targets PSMB10 in WB, IHC, Indirect ELISA applications and shows reactivity with human, pig, rat samples.

Tested Applications WB, IHC, Indirect ELISA Application Description
Tested Reactivity human, pig, rat
Immunogen PSMB10 fusion protein Ag8835 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name proteasome (prosome, macropain) subunit, beta type, 10
Synonyms beta2i, LMP10, Low molecular mass protein 10, Macropain subunit MECl 1, MECL1, Proteasome MECl 1, Proteasome subunit beta 2i, PSMB10
Calculated Molecular Weight 273 aa, 29 kDa
Observed Molecular Weight 28 kDa
GenBank Accession NumberBC017198
Gene Symbol PSMB10
Gene ID (NCBI) 5699
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP40306
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SMB10(Proteasome subunit beta type-10) is also named as LMP10, MECL1.It belongs to the peptidase T1B family and is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH.