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PSMB11 Polyclonal antibody

PSMB11 Polyclonal Antibody for WB, IHC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, FC (Intra), ELISA

Conjugate

Unconjugated

Cat no : 55143-1-AP

Print datasheet

Synonyms

Proteasome subunit beta-5t, Proteasome subunit beta type-11, EC:3.4.25.1

验证数据展示

  • WB analysis of Jurkat using 55143-1-AP

    WB analysis of Jurkat using 55143-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 55143-1-AP (PSMB11 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human thymus using 55143-1-AP

    IHC staining of human thymus using 55143-1-AP

    Immunohistochemical analysis of paraffin-embedded human thymus tissue slide using 55143-1-AP (PSMB11 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human thymus using 55143-1-AP

    IHC staining of human thymus using 55143-1-AP

    Immunohistochemical analysis of paraffin-embedded human thymus tissue slide using 55143-1-AP (PSMB11 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • FC experiment of MCF-7 using 55143-1-AP

    FC experiment of MCF-7 using 55143-1-AP

    1X10^6 MCF-7 cells were intracellularly stained with 0.2 ug Anti-Human PSMB11 (55143-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

经过测试的应用

Positive WB detected inJurkat cells
Positive IHC detected inhuman thymus tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inMCF-7 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:600
Immunohistochemistry (IHC)IHC : 1:100-1:400
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

55143-1-AP targets PSMB11 in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, FC (Intra), ELISA Application Description
Tested Reactivity human
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name proteasome (prosome, macropain) subunit, beta type, 11
Synonyms Proteasome subunit beta-5t, Proteasome subunit beta type-11, EC:3.4.25.1
Calculated Molecular Weight 33 kDa
Observed Molecular Weight 32-35 kDa
GenBank Accession NumberNM_001099780
Gene Symbol PSMB11
Gene ID (NCBI) 122706
RRIDAB_10863288
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDA5LHX3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PSMB11 belongs to the peptidase T1B family. It is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. PSMB11 has an ATP-dependent proteolytic activity. Incorporated instead of PSMB5 or PSMB8, this unit reduces the chymotrypsin-like activity of the proteasome. It plays a pivotal role in development of CD8-positive T cells. The antibody is specific to PSMB11.

实验方案

Product Specific Protocols
WB protocol for PSMB11 antibody 55143-1-APDownload protocol
IHC protocol for PSMB11 antibody 55143-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols