• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
  • Featured Product
  • KD/KO Validated

PSMB2 Monoclonal antibody, PBS Only

PSMB2 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3C5C5

Cat no : 68180-1-PBS

Print datasheet

Synonyms

HC7 I, Macropain subunit C7 I, Proteasome component C7 I, Proteasome subunit beta type 2, PSMB2

验证数据展示

  • WB analysis using 68180-1-Ig (same clone as 68180-1-PBS)

    WB analysis using 68180-1-Ig (same clone as 68180-1-PBS)

    SKOV-3 cells were subjected to SDS PAGE followed by western blot with 68180-1-Ig (PSMB2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68180-1-PBS in a different storage buffer formulation.

  • WB analysis using 68180-1-Ig (same clone as 68180-1-PBS)

    WB analysis using 68180-1-Ig (same clone as 68180-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68180-1-Ig (PSMB2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 68180-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 68180-1-Ig (same clone as 68180-1-PBS)

    WB analysis of HeLa using 68180-1-Ig (same clone as 68180-1-PBS)

    WB result of PSMB2 antibody (68180-1-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PSMB2 transfected HeLa cells. This data was developed using the same antibody clone with 68180-1-PBS in a different storage buffer formulation.

  • IF Staining of HCT 116 using 68180-1-Ig (same clone as 68180-1-PBS)

    IF Staining of HCT 116 using 68180-1-Ig (same clone as 68180-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HCT 116 cells using PSMB2 antibody (68180-1-Ig, Clone: 3C5C5 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68180-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 68180-1-Ig (same clone as 68180-1-PBS)

    IF Staining of HepG2 using 68180-1-Ig (same clone as 68180-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using PSMB2 antibody (68180-1-Ig, Clone: 3C5C5 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68180-1-PBS in a different storage buffer formulation.

  • IF Staining of PC-3 using 68180-1-Ig (same clone as 68180-1-PBS)

    IF Staining of PC-3 using 68180-1-Ig (same clone as 68180-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed PC-3 cells using PSMB2 antibody (68180-1-Ig, Clone: 3C5C5 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68180-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy PSMB2 Ready-To-Use IHC Kit. PSMB2 primary antibody included.

产品信息

68180-1-PBS targets PSMB2 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB, IF/ICC, Indirect ELISA Application Description
Tested Reactivity Human, mouse, rat
Immunogen PSMB2 fusion protein Ag7308 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name proteasome (prosome, macropain) subunit, beta type, 2
Synonyms HC7 I, Macropain subunit C7 I, Proteasome component C7 I, Proteasome subunit beta type 2, PSMB2
Calculated Molecular Weight 23 kDa
Observed Molecular Weight 23 kDa
GenBank Accession NumberBC000268
Gene Symbol PSMB2
Gene ID (NCBI) 5690
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP49721
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PSMB2(proteasome subunit beta type-2) is also named as macropain subunit C7-I, proteasome component C7-I, multicatalytic endopeptidase complex subunit C7-I and belongs to the peptidase T1B family. It is involved in an ATP/ubiquitin-dependent non-lysosomal proteolytic pathway. The up-regulation of PSMB2 may indicate the activated neuronal defensive mechanism in VAD(vitamin A depletion) brain regions, which may underlie the VAD-related psychosis behavior(PMID:21190828).