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Species Reactivity
0
Species Reactivity
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Conjugate
0
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0
Conjugate
0

验证数据展示

  • WB analysis using 68180-1-Ig (same clone as 68180-1-PBS)

    WB analysis using 68180-1-Ig (same clone as 68180-1-PBS)

    SKOV-3 cells were subjected to SDS PAGE followed by western blot with 68180-1-Ig (PSMB2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68180-1-PBS in a different storage buffer formulation.

  • WB analysis using 68180-1-Ig (same clone as 68180-1-PBS)

    WB analysis using 68180-1-Ig (same clone as 68180-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68180-1-Ig (PSMB2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 68180-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 68180-1-Ig (same clone as 68180-1-PBS)

    WB analysis of HeLa using 68180-1-Ig (same clone as 68180-1-PBS)

    WB result of PSMB2 antibody (68180-1-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PSMB2 transfected HeLa cells. This data was developed using the same antibody clone with 68180-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse liver using 68180-1-Ig (same clone as 68180-1-PBS)

    IHC staining of mouse liver using 68180-1-Ig (same clone as 68180-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 68180-1-Ig (PSMB2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68180-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PSMB2 antibody included
  • IF Staining of HCT 116 using 68180-1-Ig (same clone as 68180-1-PBS)

    IF Staining of HCT 116 using 68180-1-Ig (same clone as 68180-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HCT 116 cells using PSMB2 antibody (68180-1-Ig, Clone: 3C5C5 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68180-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 68180-1-Ig (same clone as 68180-1-PBS)

    IF Staining of HepG2 using 68180-1-Ig (same clone as 68180-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using PSMB2 antibody (68180-1-Ig, Clone: 3C5C5 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68180-1-PBS in a different storage buffer formulation.

  • IF Staining of PC-3 using 68180-1-Ig (same clone as 68180-1-PBS)

    IF Staining of PC-3 using 68180-1-Ig (same clone as 68180-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed PC-3 cells using PSMB2 antibody (68180-1-Ig, Clone: 3C5C5 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68180-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

PSMB2 Monoclonal antibody, PBS Only

PSMB2 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 68180-1-PBS

产品说明书

CloneNo. 3C5C5

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

3C5C5, Macropain subunit C7-I, Multicatalytic endopeptidase complex subunit C7-I, Proteasome component C7-I, Proteasome subunit beta type-2

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy PSMB2 Ready-To-Use IHC Kit. PSMB2 primary antibody included.

产品信息

68180-1-PBS targets PSMB2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 PSMB2 fusion protein Ag7308 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 proteasome (prosome, macropain) subunit, beta type, 2
别名 3C5C5, Macropain subunit C7-I, Multicatalytic endopeptidase complex subunit C7-I, Proteasome component C7-I, Proteasome subunit beta type-2
计算分子量 23 kDa
观测分子量 23 kDa
GenBank蛋白编号BC000268
基因名称 PSMB2
Gene ID (NCBI) 5690
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP49721
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PSMB2(proteasome subunit beta type-2) is also named as macropain subunit C7-I, proteasome component C7-I, multicatalytic endopeptidase complex subunit C7-I and belongs to the peptidase T1B family. It is involved in an ATP/ubiquitin-dependent non-lysosomal proteolytic pathway. The up-regulation of PSMB2 may indicate the activated neuronal defensive mechanism in VAD(vitamin A depletion) brain regions, which may underlie the VAD-related psychosis behavior(PMID:21190828).