验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, HepG2 cells, Jurkat cells, L02 cells |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy PSMB5 Ready-To-Use IHC Kit. PSMB5 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
19178-1-AP targets PSMB5 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse |
| 免疫原 | PSMB5 fusion protein Ag5828 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | proteasome (prosome, macropain) subunit, beta type, 5 |
| 别名 | X, Proteasome subunit beta type-5, MB1, LMPX, EC:3.4.25.1 |
| 计算分子量 | 263 aa, 28 kDa |
| 观测分子量 | 22 kDa |
| GenBank蛋白编号 | BC057840 |
| 基因名称 | PSMB5 |
| Gene ID (NCBI) | 5693 |
| RRID | AB_10926385 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P28074 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PSMB5(Proteasome subunit beta type-5) is also named as LMPX, MB1, X and belongs to the peptidase T1B family.The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH.RNAi-mediated silencing of.It has 2 isoforms produced by alternative splicing.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PSMB5 antibody 19178-1-AP | Download protocol |
| IHC protocol for PSMB5 antibody 19178-1-AP | Download protocol |
| IF protocol for PSMB5 antibody 19178-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun The deubiquitinase OTUD1 regulates immunoglobulin production and proteasome inhibitor sensitivity in multiple myeloma | ||
Cancer Lett Combinations of ivermectin with proteasome inhibitors induce synergistic lethality in multiple myeloma | ||
Front Pharmacol Selective Inhibition of the Immunoproteasome β5i Prevents PTEN Degradation and Attenuates Cardiac Hypertrophy. | ||
J Cancer Cytoplasm-localized SIRT1 downregulation attenuates apoptosis and cell cycle arrest in cisplatin-resistant lung cancer A549 cells. | ||
Biochem Pharmacol Melatonin inhibits angiotensin II-induced atrial fibrillation through preventing degradation of Ang II Type I Receptor-Associated Protein (ATRAP) |