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  • Featured Product
  • KD/KO Validated

PSMB5 Monoclonal antibody, PBS Only

PSMB5 Monoclonal Antibody for WB,Indirect ELISA,IHC,IF

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

WB,Indirect ELISA,IHC,IF

Conjugate

Unconjugated

CloneNo.

2E9H1

Cat No : 67959-1-PBS

Print datasheet

Synonyms

LMPX, Macropain epsilon chain, MB1, Proteasome chain 6, Proteasome epsilon chain, Proteasome subunit beta type 5, Proteasome subunit MB1, Proteasome subunit X, PSMB5, X

验证数据展示

  • WB analysis using 67959-1-Ig (same clone as 67959-1-PBS)

    WB analysis using 67959-1-Ig (same clone as 67959-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67959-1-Ig (PSMB5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67959-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 67959-1-Ig (same clone as 67959-1-PBS)

    IHC staining of human breast cancer using 67959-1-Ig (same clone as 67959-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67959-1-Ig (PSMB5 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67959-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PSMB5 antibody included
  • IF Staining of HeLa using 67959-1-Ig (same clone as 67959-1-PBS)

    IF Staining of HeLa using 67959-1-Ig (same clone as 67959-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PSMB5 antibody (67959-1-Ig, Clone: 2E9H1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67959-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 67959-1-Ig (same clone as 67959-1-PBS)

    WB analysis of HeLa using 67959-1-Ig (same clone as 67959-1-PBS)

    WB result of PSMB5 antibody (67959-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PSMB5 transfected HeLa cells. This data was developed using the same antibody clone with 67959-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy PSMB5 Ready-To-Use IHC Kit. PSMB5 primary antibody included.

产品信息

67959-1-PBS targets PSMB5 in WB, IF, IHC, Indirect ELISA applications and shows reactivity with Human samples.

Tested Applications WB,Indirect ELISA,IHC,IF Application Description
Tested Reactivity Human
Immunogen PSMB5 fusion protein Ag5828 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name proteasome (prosome, macropain) subunit, beta type, 5
Synonyms LMPX, Macropain epsilon chain, MB1, Proteasome chain 6, Proteasome epsilon chain, Proteasome subunit beta type 5, Proteasome subunit MB1, Proteasome subunit X, PSMB5, X
Calculated Molecular Weight 263 aa, 28 kDa
Observed Molecular Weight22 kDa
GenBank Accession NumberBC057840
Gene Symbol PSMB5
Gene ID (NCBI) 5693
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP28074
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

PSMB5(Proteasome subunit beta type-5) is also named as LMPX, MB1, X and belongs to the peptidase T1B family.The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH.RNAi-mediated silencing of.It has 2 isoforms produced by alternative splicing.