PSMB6 Polyclonal antibody

PSMB6 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 11684-2-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

Y, Proteasome subunit beta type-6, LMPY, EC:3.4.25.1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inHeLa cells, Caco-2 cells, fetal human brain tissue, human liver tissue, mouse brain tissue, rat brain tissue
Positive IHC detected inhuman colon cancer tissue, human gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy PSMB6 Ready-To-Use IHC Kit. PSMB6 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 5 publications below

产品信息

11684-2-AP targets PSMB6 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性human, mouse, rat
免疫原 PSMB6 fusion protein Ag2296 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 proteasome (prosome, macropain) subunit, beta type, 6
别名 Y, Proteasome subunit beta type-6, LMPY, EC:3.4.25.1
计算分子量 239 aa, 25 kDa
观测分子量 25 kDa
GenBank蛋白编号BC000835
基因名称 PSMB6
Gene ID (NCBI) 5694
RRIDAB_2172199
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP28072
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PSMB6(Proteasome subunit beta type-6) is also named as LMPY, Y and belongs to the peptidase T1B family.The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH.It may also catalyzes basal processing of intracellular antigens.It can be up-regulated in anaplastic thyroid cancer cell lines and down-regulated by IFNG/IFN-gamma (at protein level)(PMID:8066462;15613457).

实验方案

Product Specific Protocols
WB protocol for PSMB6 antibody 11684-2-APDownload protocol
IHC protocol for PSMB6 antibody 11684-2-APDownload protocol
IF protocol for PSMB6 antibody 11684-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
ratWB

Cell Physiol Biochem

The Favored Mechanism for Coping with Acute Cold Stress: Upregulation of miR-210 in Rats.

Authors - Wenjin Guo
mouseWB

J Biol Chem

Hepatocyte-specific Wtap deficiency promotes hepatocellular carcinoma by activating GRB2/ERK depending on downregulation of proteasome-related genes

Authors - Xinzhi Li
humanWB

Front Genet

Immune regulation and prognostic prediction model establishment and validation of PSMB6 in lung adenocarcinoma

Authors - Haiyang Zhao
mouseWB

Biochem Pharmacol

Melatonin inhibits angiotensin II-induced atrial fibrillation through preventing degradation of Ang II Type I Receptor-Associated Protein (ATRAP)

Authors - Xin Xie
mouseWB

JHEP Rep

Downregulation of hepatic METTL3 contributes to APAP-induced liver injury in mice

Authors - Chunhong Liu
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