验证数据展示
经过测试的应用
Positive WB detected in | HeLa cells, Caco-2 cells, fetal human brain tissue, human liver tissue, mouse brain tissue, rat brain tissue |
Positive IHC detected in | human colon cancer tissue, human gliomas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HeLa cells |
推荐稀释比
应用 | 推荐稀释比 |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 5 publications below |
产品信息
11684-2-AP targets PSMB6 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
文献引用应用 | WB |
经测试反应性 | human, mouse, rat |
文献引用反应性 | human, mouse, rat |
免疫原 | PSMB6 fusion protein Ag2296 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | proteasome (prosome, macropain) subunit, beta type, 6 |
别名 | Y, Proteasome subunit beta type-6, LMPY, EC:3.4.25.1 |
计算分子量 | 239 aa, 25 kDa |
观测分子量 | 25 kDa |
GenBank蛋白编号 | BC000835 |
基因名称 | PSMB6 |
Gene ID (NCBI) | 5694 |
RRID | AB_2172199 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | P28072 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PSMB6(Proteasome subunit beta type-6) is also named as LMPY, Y and belongs to the peptidase T1B family.The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH.It may also catalyzes basal processing of intracellular antigens.It can be up-regulated in anaplastic thyroid cancer cell lines and down-regulated by IFNG/IFN-gamma (at protein level)(PMID:8066462;15613457).
实验方案
Product Specific Protocols | |
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WB protocol for PSMB6 antibody 11684-2-AP | Download protocol |
IHC protocol for PSMB6 antibody 11684-2-AP | Download protocol |
IF protocol for PSMB6 antibody 11684-2-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Cell Physiol Biochem The Favored Mechanism for Coping with Acute Cold Stress: Upregulation of miR-210 in Rats. | ||
J Biol Chem Hepatocyte-specific Wtap deficiency promotes hepatocellular carcinoma by activating GRB2/ERK depending on downregulation of proteasome-related genes | ||
Front Genet Immune regulation and prognostic prediction model establishment and validation of PSMB6 in lung adenocarcinoma | ||
Biochem Pharmacol Melatonin inhibits angiotensin II-induced atrial fibrillation through preventing degradation of Ang II Type I Receptor-Associated Protein (ATRAP) | ||
JHEP Rep Downregulation of hepatic METTL3 contributes to APAP-induced liver injury in mice |