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验证数据展示

  • WB analysis using 30283-1-AP

    WB analysis using 30283-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30283-1-AP (PSMB7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis using 30283-1-AP

    WB analysis using 30283-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30283-1-AP (PSMB7 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 30283-1-AP

    IHC staining of human colon cancer using 30283-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 30283-1-AP (PSMB7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PSMB7 antibody included
  • IHC staining of human colon cancer using 30283-1-AP

    IHC staining of human colon cancer using 30283-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 30283-1-AP (PSMB7 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PSMB7 antibody included

PSMB7 Polyclonal antibody

PSMB7 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 30283-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human, mouse, rat

应用

WB, IHC, ELISA

别名

Macropain chain Z, Proteasome subunit beta type 7, Proteasome subunit Z, PSMB7, Z

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, MCF-7 cells, HepG2 cells, mouse skeletal muscle tissue, rat skeletal muscle tissue, mouse kidney tissue, rat kidney tissue
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy PSMB7 Ready-To-Use IHC Kit. PSMB7 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30283-1-AP targets PSMB7 in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 Human, mouse, rat
免疫原 PSMB7 fusion protein Ag32201 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 proteasome (prosome, macropain) subunit, beta type, 7
别名 Macropain chain Z, Proteasome subunit beta type 7, Proteasome subunit Z, PSMB7, Z
计算分子量29.9 kDa
观测分子量28-30 kDa
GenBank蛋白编号BC000509
基因名称 PSMB7
Gene ID (NCBI) 5695
RRIDAB_3086285
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ99436
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. PSMB7 protein is a member of the proteasome B-type family, also known as the T1B family, and is a 20S core beta subunit in the proteasome. Expression of this catalytic subunit is downregulated by gamma interferon, and proteolytic processing is required to generate a mature subunit. Component of the 20S core proteasome complex are involved in the proteolytic degradation of most intracellular proteins. PSMB7 displays a trypsin-like activity.(PMID:15244466, PMID:27176742, PMID: 8610016)

实验方案

Product Specific Protocols
WB protocol for PSMB7 antibody 30283-1-APDownload protocol
IHC protocol for PSMB7 antibody 30283-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols