验证数据展示
经过测试的应用
| Positive WB detected in | HL-60 cells, rat spleen tissue, mouse kidney tissue, mouse liver tissue, mouse lung tissue, mouse spleen tissue, rat liver tissue, Raji cells |
| Positive IP detected in | Raji cells |
| Positive IHC detected in | human spleen tissue, human tonsillitis tissue, human renal cell carcinoma tissue, mouse bladder tissue, mouse stomach tissue, rat stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Planning an IHC experiment? We recommend our IHCeasy PSMB9 Ready-To-Use IHC Kit. PSMB9 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
14544-1-AP targets PSMB9 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | PSMB9 fusion protein Ag6048 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | proteasome (prosome, macropain) subunit, beta type, 9 (large multifunctional peptidase 2) |
| 别名 | LMP2, EC:3.4.25.1, RING12, PSMB6i, Proteasome subunit beta-1i |
| 计算分子量 | 23 kDa |
| 观测分子量 | 20-23 kDa |
| GenBank蛋白编号 | BC065513 |
| 基因名称 | PSMB9 |
| Gene ID (NCBI) | 5698 |
| RRID | AB_2268925 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P28065 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PSMB9, also named as LMP2, PSMB6i and RING12, belongs to the peptidase T1B family. The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. Replacement of PSMB6 by PSMB9 increases the capacity of the immunoproteasome to cleave model peptides after hydrophobic and basic residues. High expression of PSMB9 associated with the poor prognosis of patients with NPC.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PSMB9 antibody 14544-1-AP | Download protocol |
| IHC protocol for PSMB9 antibody 14544-1-AP | Download protocol |
| IF protocol for PSMB9 antibody 14544-1-AP | Download protocol |
| IP protocol for PSMB9 antibody 14544-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Mol Cell DNA damage promotes HLA class I presentation by stimulating a pioneer round of translation-associated antigen production.
| ||
Cell Res In-cell infection: a novel pathway for Epstein-Barr virus infection mediated by cell-in-cell structures. | ||
EMBO J Proteasomal degradation within endocytic organelles mediates antigen cross-presentation. | ||
Cancer Lett Deciphering the tumor-suppressive role of PSMB9 in melanoma through multi-omics and single-cell transcriptome analyses | ||
Cell Rep ECM29/proteasome-mediated self-antigen generation by CNS-resident neuroglia promotes regulatory T cell activation |
