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验证数据展示

  • WB analysis using 67748-1-Ig

    WB analysis using 67748-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67748-1-Ig (PSMB9 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • WB analysis of rat spleen using 67748-1-Ig

    WB analysis of rat spleen using 67748-1-Ig

    rat spleen tissue were subjected to SDS PAGE followed by western blot with 67748-1-Ig (PSMB9 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 67748-1-Ig

    IHC staining of human colon cancer using 67748-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67748-1-Ig (PSMB9 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PSMB9 antibody included

PSMB9 Monoclonal antibody

PSMB9 Monoclonal Antibody for WB, IHC, ELISA
Cat No. 67748-1-Ig

产品说明书

CloneNo. 1C7C8

宿主/亚型

Mouse / IgG2a

种属反应性

Human, rat

应用

WB, IHC, ELISA

别名

beta1i, LMP2, Low molecular mass protein 2, Macropain chain 7, Proteasome chain 7, Proteasome subunit beta 1i, Proteasome subunit beta type 9, PSMB6i, PSMB9, RING12

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inRaji cells, rat spleen tissue, Ramos cells, Daudi cells, HL-60 cells, THP-1 cells
Positive IHC detected inhuman colon cancer tissue, human spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy PSMB9 Ready-To-Use IHC Kit. PSMB9 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:300-1:1200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67748-1-Ig targets PSMB9 in WB, IHC, ELISA applications and shows reactivity with Human, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 Human, rat
免疫原 PSMB9 fusion protein Ag6802 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 proteasome (prosome, macropain) subunit, beta type, 9 (large multifunctional peptidase 2)
别名 beta1i, LMP2, Low molecular mass protein 2, Macropain chain 7, Proteasome chain 7, Proteasome subunit beta 1i, Proteasome subunit beta type 9, PSMB6i, PSMB9, RING12
计算分子量 23 kDa
观测分子量20-23 kDa
GenBank蛋白编号BC065513
基因名称 PSMB9
Gene ID (NCBI) 5698
RRIDAB_2918517
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP28065
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PSMB9 antibody 67748-1-IgDownload protocol
IHC protocol for PSMB9 antibody 67748-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols