- Featured Product
- KD/KO Validated
HOP2 Polyclonal antibody
HOP2 Polyclonal Antibody for IF/ICC, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, rat, mouse
Applications
IF/ICC, IHC, IP, WB, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | HEK-293 cells, K-562 cells, Jurkat cells |
Positive IP detected in | Jurkat cells |
Positive IHC detected in | mouse testis tissue, rat testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HepG2 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:400-1:1600 |
Immunofluorescence (IF) | IF : 1:10-1:100 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
11339-1-AP targets HOP2 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, rat, mouse samples.
Tested Applications | IF/ICC, IHC, IP, WB, ELISA Application Description |
Cited Applications | WB, IF |
Tested Reactivity | human, rat, mouse |
Cited Reactivity | human, mouse |
Immunogen | HOP2 fusion protein Ag1883 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | PSMC3 interacting protein |
Synonyms | GT198, HOP2, HUMGT198A, PSMC3 interacting protein, PSMC3IP, TBP 1 interacting protein, TBPIP |
Calculated Molecular Weight | 25 kDa |
Observed Molecular Weight | 24 kDa and 29 kDa |
GenBank Accession Number | BC008792 |
Gene Symbol | PSMC3IP |
Gene ID (NCBI) | 29893 |
RRID | AB_2172642 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q9P2W1 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
---|---|
WB protocol for HOP2 antibody 11339-1-AP | Download protocol |
IHC protocol for HOP2 antibody 11339-1-AP | Download protocol |
IF protocol for HOP2 antibody 11339-1-AP | Download protocol |
IP protocol for HOP2 antibody 11339-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Cell Interchromosomal Homology Searches Drive Directional ALT Telomere Movement and Synapsis.
| ||
Nucleic Acids Res RNase H1 facilitates recombinase recruitment by degrading DNA-RNA hybrids during meiosis | ||
Oncotarget A study of meiomitosis and novel pathways of genomic instability in cutaneous T-cell lymphomas (CTCL). |