验证数据展示
经过测试的应用
| Positive WB detected in | mouse brain tissue, rat brain tissue |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | SH-SY5Y cells, HepG2 cells |
Planning an IHC experiment? We recommend our IHCeasy PSMC4 Ready-To-Use IHC Kit. PSMC4 primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
11389-1-AP targets PSMC4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, IHC, IF/ICC, ELISA Application Description |
| Cited Applications | WB, IHC, IF |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Immunogen | PSMC4 fusion protein Ag1943 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | proteasome (prosome, macropain) 26S subunit, ATPase, 4 |
| Synonyms | Tat-binding protein 7, S6, MIP224, MB67-interacting protein, 26S proteasome regulatory subunit 6B |
| Calculated Molecular Weight | 418 aa, 47 kDa |
| Observed Molecular Weight | 43-50 kDa |
| GenBank Accession Number | BC014488 |
| Gene Symbol | PSMC4 |
| Gene ID (NCBI) | 5704 |
| RRID | AB_2300373 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P43686 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PSMC4(proteasome (prosome, macropain) 26S subunit, ATPase, 4) is also named as TBP7, S6, TBP7, MIP224 and belongs to the AAA ATPase family. The 26S protease is involved in the ATP-dependent degradation of ubiquitinated proteins. The predicted 458-amino acid TBP7 protein is 57% identical to TBP1 and they can form heterodimers(PMID:8419915).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PSMC4 antibody 11389-1-AP | Download protocol |
| IHC protocol for PSMC4 antibody 11389-1-AP | Download protocol |
| IF protocol for PSMC4 antibody 11389-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
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