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验证数据展示

  • WB analysis using 14752-1-AP

    WB analysis using 14752-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14752-1-AP (PSMC5 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 14752-1-AP

    IHC staining of human liver cancer using 14752-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14752-1-AP (PSMC5 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver cancer using 14752-1-AP

    IHC staining of human liver cancer using 14752-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14752-1-AP (PSMC5 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

PSMC5 Polyclonal antibody

PSMC5 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 14752-1-AP

Print datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

p45, p45/SUG, Proteasome subunit p45, PSMC5, Rpt6, S8, SUG1, TBP10, TRIP1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inA549 cells, NIH/3T3 cells, HEK-293 cells, Jurkat cells, mouse kidney tissue
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14752-1-AP targets PSMC5 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen PSMC5 fusion protein Ag6497 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name proteasome (prosome, macropain) 26S subunit, ATPase, 5
Synonyms p45, p45/SUG, Proteasome subunit p45, PSMC5, Rpt6, S8, SUG1, TBP10, TRIP1
Calculated Molecular Weight 46 kDa
Observed Molecular Weight 45-49 kDa
GenBank Accession NumberBC002367
Gene Symbol PSMC5
Gene ID (NCBI) 5705
RRIDAB_2878077
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP62195
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PSMC5 antibody 14752-1-APDownload protocol
IHC protocol for PSMC5 antibody 14752-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols