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验证数据展示

  • WB analysis using 14752-1-AP

    WB analysis using 14752-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14752-1-AP (PSMC5 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 14752-1-AP

    IHC staining of human liver cancer using 14752-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14752-1-AP (PSMC5 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver cancer using 14752-1-AP

    IHC staining of human liver cancer using 14752-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14752-1-AP (PSMC5 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

PSMC5 Polyclonal antibody

PSMC5 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 14752-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

p45, p45/SUG, Proteasome subunit p45, PSMC5, Rpt6, S8, SUG1, TBP10, TRIP1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA549 cells, NIH/3T3 cells, HEK-293 cells, Jurkat cells, mouse kidney tissue
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14752-1-AP targets PSMC5 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 PSMC5 fusion protein Ag6497 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 proteasome (prosome, macropain) 26S subunit, ATPase, 5
别名 p45, p45/SUG, Proteasome subunit p45, PSMC5, Rpt6, S8, SUG1, TBP10, TRIP1
计算分子量 46 kDa
观测分子量 45-49 kDa
GenBank蛋白编号BC002367
基因名称 PSMC5
Gene ID (NCBI) 5705
RRIDAB_2878077
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP62195
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PSMC5 antibody 14752-1-APDownload protocol
IHC protocol for PSMC5 antibody 14752-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols