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验证数据展示

  • WB analysis using 83521-2-RR

    WB analysis using 83521-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83521-2-RR (PSMD1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of human intrahepatic cholangiocarcinoma using 83521-2-RR

    IHC staining of human intrahepatic cholangiocarcinoma using 83521-2-RR

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 83521-2-RR (PSMD1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human intrahepatic cholangiocarcinoma using 83521-2-RR

    IHC staining of human intrahepatic cholangiocarcinoma using 83521-2-RR

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 83521-2-RR (PSMD1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • FC experiment of MCF-7 using 83521-2-RR

    FC experiment of MCF-7 using 83521-2-RR

    1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug PSMD1 Recombinant antibody (83521-2-RR, Clone:240458B3) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Affinity and Kinetic Characterization of 83521-2-RR

    Affinity and Kinetic Characterization of 83521-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83521-2-RR against Human PSMD1 were performed. The affinity constant is below 1 pM.

PSMD1 Recombinant antibody

PSMD1 Uni-rAbTM Recombinant Antibody for WB, IHC, FC (Intra), ELISA
Cat No. 83521-2-RR

产品说明书

CloneNo. 240458B3

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, FC (Intra), ELISA

别名

PSMD 1, 26S proteasome non-ATPase regulatory subunit 1, 240458B3

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inJurkat cells, SKOV-3 cells, MCF-7 cells, mouse brain tissue, mouse testis tissue, rat brain tissue
Positive IHC detected inhuman intrahepatic cholangiocarcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inMCF-7 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83521-2-RR targets PSMD1 in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, FC (Intra), ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 PSMD1 fusion protein Ag12009 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 proteasome (prosome, macropain) 26S subunit, non-ATPase, 1
别名 PSMD 1, 26S proteasome non-ATPase regulatory subunit 1, 240458B3
计算分子量 953 aa, 106 kDa
观测分子量106 kDa
GenBank蛋白编号BC094720
基因名称 PSMD1
Gene ID (NCBI) 5707
RRIDAB_3671147
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ99460
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PSMD1 is a component of the 26S proteasome and contributes to its function regulation. PSMD1 performs an important role in the regulation of numerous cellular processes through the degradation of proteins targeted for breakdown through ubiquitin. PSMD1 has been found to be closely related to a variety of cancers such as lung adenocarcinoma and breast cancer.

实验方案

Product Specific Protocols
WB protocol for PSMD1 antibody 83521-2-RRDownload protocol
IHC protocol for PSMD1 antibody 83521-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols