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验证数据展示

  • WB analysis using 83521-4-RR

    WB analysis using 83521-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83521-4-RR (PSMD1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human intrahepatic cholangiocarcinoma using 83521-4-RR

    IHC staining of human intrahepatic cholangiocarcinoma using 83521-4-RR

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 83521-4-RR (PSMD1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human intrahepatic cholangiocarcinoma using 83521-4-RR

    IHC staining of human intrahepatic cholangiocarcinoma using 83521-4-RR

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 83521-4-RR (PSMD1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 83521-4-RR

    IF Staining of HeLa using 83521-4-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PSMD1 antibody (83521-4-RR, Clone: 240458C9 ) at dilution of 1:300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • FC experiment of MCF-7 using 83521-4-RR

    FC experiment of MCF-7 using 83521-4-RR

    1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug PSMD1 Recombinant antibody (83521-4-RR, Clone:240458C9) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Affinity and Kinetic Characterization of 83521-4-RR

    Affinity and Kinetic Characterization of 83521-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83521-4-RR against Human PSMD1 were performed. The affinity constant is 74.2 pM.

PSMD1 Recombinant antibody

PSMD1 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA
Cat No. 83521-4-RR
CloneNo. 240458C9

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Synonyms

PSMD 1, 26S proteasome non-ATPase regulatory subunit 1, 240458C9

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive WB detected inJurkat cells, SKOV-3 cells, MCF-7 cells, mouse brain tissue, mouse testis tissue, rat brain tissue
Positive IHC detected inhuman intrahepatic cholangiocarcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inMCF-7 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:150-1:600
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83521-4-RR targets PSMD1 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen PSMD1 fusion protein Ag12009 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name proteasome (prosome, macropain) 26S subunit, non-ATPase, 1
Synonyms PSMD 1, 26S proteasome non-ATPase regulatory subunit 1, 240458C9
Calculated Molecular Weight 953 aa, 106 kDa
Observed Molecular Weight106 kDa
GenBank Accession NumberBC094720
Gene Symbol PSMD1
Gene ID (NCBI) 5707
RRIDAB_3671148
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ99460
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PSMD1 is a component of the 26S proteasome and contributes to its function regulation. PSMD1 performs an important role in the regulation of numerous cellular processes through the degradation of proteins targeted for breakdown through ubiquitin. PSMD1 has been found to be closely related to a variety of cancers such as lung adenocarcinoma and breast cancer.

实验方案

Product Specific Protocols
WB protocol for PSMD1 antibody 83521-4-RRDownload protocol
IHC protocol for PSMD1 antibody 83521-4-RRDownload protocol
IF protocol for PSMD1 antibody 83521-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols