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验证数据展示

  • WB analysis using 11412-1-AP (same clone as 11412-1-PBS)

    WB analysis using 11412-1-AP (same clone as 11412-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 11412-1-AP (PSMD12 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11412-1-PBS in a different storage buffer formulation.

  • IHC staining of human gliomas using 11412-1-AP (same clone as 11412-1-PBS)

    IHC staining of human gliomas using 11412-1-AP (same clone as 11412-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 11412-1-AP (PSMD12 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11412-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PSMD12 antibody included
  • Featured Product
  • KD/KO验证

PSMD12 Polyclonal antibody, PBS Only

PSMD12 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 11412-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy PSMD12 Ready-To-Use IHC Kit. PSMD12 primary antibody included.

产品信息

11412-1-PBS targets PSMD12 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 PSMD12 fusion protein Ag1957 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 proteasome (prosome, macropain) 26S subunit, non-ATPase, 12
别名
计算分子量 456 aa, 53 kDa
观测分子量 50-55 kDa
GenBank蛋白编号BC019062
基因名称 PSMD12
Gene ID (NCBI) 5718
RRIDAB_10640803
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO00232
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Proteasome 26S subunit, non-ATPase 12 (PSMD12; also known as RPN5) was first discovered in fission yeast. It is a non-ATPase subunit of the 19S regulator of the 26S proteasome complex, which is involved in regulating the cell cycle, repairing DNA damage, and regulating apoptosis by mediating the internal protein balance through the removal of misfolded proteins or damaged intracellular substructures. In addition, PSMD12 contributes to tumor cell growth and apoptosis in breast cancer. (PMID: 33987398)