• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis of pig liver using 67682-1-Ig

    WB analysis of pig liver using 67682-1-Ig

    pig liver tissue were subjected to SDS PAGE followed by western blot with 67682-1-Ig (PSMD5 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 67682-1-Ig

    IHC staining of human colon cancer using 67682-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67682-1-Ig (PSMD5 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PSMD5 antibody included
  • IHC staining of human colon cancer using 67682-1-Ig

    IHC staining of human colon cancer using 67682-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67682-1-Ig (PSMD5 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PSMD5 antibody included
  • IF Staining of A431 using 67682-1-Ig

    IF Staining of A431 using 67682-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using PSMD5 antibody (67682-1-Ig, Clone: 1D5F7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red).

PSMD5 Monoclonal antibody

PSMD5 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 67682-1-Ig

产品说明书

CloneNo. 1D5F7

宿主/亚型

Mouse / IgG3

种属反应性

Human, pig, rat, rabbit, mouse

应用

WB, IHC, IF/ICC, ELISA

别名

26S protease subunit S5 basic, 26S proteasome subunit S5B, KIAA0072, PSMD5, S5B

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inpig liver tissue, rabbit liver tissue, rat liver tissue, K-562 cells, HCT 116 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells
Planning an IHC experiment? We recommend our IHCeasy PSMD5 Ready-To-Use IHC Kit. PSMD5 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67682-1-Ig targets PSMD5 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, pig, rat, rabbit, mouse samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 Human, pig, rat, rabbit, mouse
免疫原 PSMD5 fusion protein Ag19298 种属同源性预测
宿主/亚型 Mouse / IgG3
抗体类别 Monoclonal
产品类型 Antibody
全称 proteasome (prosome, macropain) 26S subunit, non-ATPase, 5
别名 26S protease subunit S5 basic, 26S proteasome subunit S5B, KIAA0072, PSMD5, S5B
计算分子量 504 aa, 56 kDa
观测分子量50 kDa
GenBank蛋白编号BC014478
基因名称 PSMD5
Gene ID (NCBI) 5711
RRIDAB_2882875
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ16401
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PSMD5 antibody 67682-1-IgDownload protocol
IHC protocol for PSMD5 antibody 67682-1-IgDownload protocol
IF protocol for PSMD5 antibody 67682-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols