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PSMD5 Monoclonal antibody

PSMD5 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG3

Reactivity

Human, pig, rat, rabbit, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1D5F7

Cat no : 67682-1-Ig

Print datasheet

Synonyms

26S protease subunit S5 basic, 26S proteasome subunit S5B, KIAA0072, PSMD5, S5B

验证数据展示

  • WB analysis of pig liver using 67682-1-Ig

    WB analysis of pig liver using 67682-1-Ig

    pig liver tissue were subjected to SDS PAGE followed by western blot with 67682-1-Ig (PSMD5 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 67682-1-Ig

    IHC staining of human colon cancer using 67682-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67682-1-Ig (PSMD5 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PSMD5 antibody included
  • IHC staining of human colon cancer using 67682-1-Ig

    IHC staining of human colon cancer using 67682-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67682-1-Ig (PSMD5 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PSMD5 antibody included
  • IF Staining of A431 using 67682-1-Ig

    IF Staining of A431 using 67682-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using PSMD5 antibody (67682-1-Ig, Clone: 1D5F7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red).

经过测试的应用

Positive WB detected inpig liver tissue, rabbit liver tissue, rat liver tissue, K-562 cells, HCT 116 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells
Planning an IHC experiment? We recommend our IHCeasy PSMD5 Ready-To-Use IHC Kit. PSMD5 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67682-1-Ig targets PSMD5 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, pig, rat, rabbit, mouse samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity Human, pig, rat, rabbit, mouse
Immunogen PSMD5 fusion protein Ag19298 种属同源性预测
Host / Isotype Mouse / IgG3
Class Monoclonal
Type Antibody
Full Name proteasome (prosome, macropain) 26S subunit, non-ATPase, 5
Synonyms 26S protease subunit S5 basic, 26S proteasome subunit S5B, KIAA0072, PSMD5, S5B
Calculated Molecular Weight 504 aa, 56 kDa
Observed Molecular Weight50 kDa
GenBank Accession NumberBC014478
Gene Symbol PSMD5
Gene ID (NCBI) 5711
RRIDAB_2882875
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ16401
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PSMD5 antibody 67682-1-IgDownload protocol
IHC protocol for PSMD5 antibody 67682-1-IgDownload protocol
IF protocol for PSMD5 antibody 67682-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols