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PSMD6 Polyclonal antibody

PSMD6 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 12539-1-AP

Print datasheet

Synonyms

KIAA0107, p42A, p44S10, PFAAP4, PSMD6, Rpn7, S10, SGA 113M

验证数据展示

  • WB analysis using 12539-1-AP

    WB analysis using 12539-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12539-1-AP (PSMD6 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse testis using 12539-1-AP

    IHC staining of mouse testis using 12539-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 12539-1-AP (PSMD6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse kidney using 12539-1-AP

    IHC staining of mouse kidney using 12539-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 12539-1-AP (PSMD6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 12539-1-AP

    IHC staining of mouse brain using 12539-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12539-1-AP (PSMD6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IP experiment of K-562 using 12539-1-AP

    IP experiment of K-562 using 12539-1-AP

    IP result of anti-PSMD6 (IP:12539-1-AP, 3ug; Detection:12539-1-AP 1:500) with K-562 cells lysate 1500ug.

经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, Jurkat cells
Positive IP detected inK-562 cells
Positive IHC detected inmouse testis tissue, mouse brain tissue, mouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

12539-1-AP targets PSMD6 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IP, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen PSMD6 fusion protein Ag3251 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name proteasome (prosome, macropain) 26S subunit, non-ATPase, 6
Synonyms KIAA0107, p42A, p44S10, PFAAP4, PSMD6, Rpn7, S10, SGA 113M
Calculated Molecular Weight 389 aa, 46 kDa
Observed Molecular Weight 46 kDa
GenBank Accession NumberBC012369
Gene Symbol PSMD6
Gene ID (NCBI) 9861
RRIDAB_2170789
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ15008
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The 26 S proteasome is a 2.5-MDa molecular machine that degrades ubiquitinated proteins in eukaryotic cells. It consists of a proteolytic core particle and two 19 S regulatory particles (RPs) composed of 6 ATPase (RPT) and 13 non-ATPase (RPN) subunits. PSMD6 gene encodes 26S proteasome regulatory subunit RPN7, the characteristic of this protein is not well known to date.

实验方案

Product Specific Protocols
WB protocol for PSMD6 antibody 12539-1-APDownload protocol
IHC protocol for PSMD6 antibody 12539-1-APDownload protocol
IP protocol for PSMD6 antibody 12539-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cells

The Identification of Nuclear FMRP Isoform Iso6 Partners

Authors - Nassim Ledoux
View Article