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验证数据展示

  • WB analysis of A549 using 26922-1-AP

    WB analysis of A549 using 26922-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 26922-1-AP (PSMD9 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis of A549 using 26922-1-AP

    WB analysis of A549 using 26922-1-AP

    WB result of PSMD9 antibody (26922-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PSMD9 transfected A549 cells.

  • IHC staining of human breast cancer using 26922-1-AP

    IHC staining of human breast cancer using 26922-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 26922-1-AP (PSMD9 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human breast cancer using 26922-1-AP

    IHC staining of human breast cancer using 26922-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 26922-1-AP (PSMD9 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IP experiment of A549 using 26922-1-AP

    IP experiment of A549 using 26922-1-AP

    IP result of anti-PSMD9 (IP:26922-1-AP, 4ug; Detection:26922-1-AP 1:400) with A549 cells lysate 1330 ug.

  • IF Staining of U2OS using 26922-1-AP

    IF Staining of U2OS using 26922-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using PSMD9 antibody (26922-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • FC experiment of HeLa using 26922-1-AP

    FC experiment of HeLa using 26922-1-AP

    1x10^6 HeLa cells were intracellularly stained with 0.8 ug Anti-Human PSMD9 (26922-1-AP)(red), or 0.8 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IF Staining of A549 using 26922-1-AP

    IF Staining of A549 using 26922-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using PSMD9 antibody (26922-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

  • Featured Product
  • KD/KO验证

PSMD9 Polyclonal antibody

PSMD9 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA
Cat No. 26922-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

别名

Rpn4, p27, 26S proteasome non-ATPase regulatory subunit 9

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inA549 cells, MCF-7 cells, MDA-MB-453s cells, HepG2 cells, Jurkat cells, mouse spleen tissue
Positive IP detected inA549 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU2OS cells, A549 cells
Positive FC (Intra) detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26922-1-AP targets PSMD9 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description
经测试反应性 human, mouse
免疫原 PSMD9 fusion protein Ag25638 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 proteasome (prosome, macropain) 26S subunit, non-ATPase, 9
别名 Rpn4, p27, 26S proteasome non-ATPase regulatory subunit 9
计算分子量 27 kDa
观测分子量 25-30 kDa
GenBank蛋白编号BC004213
基因名称 PSMD9
Gene ID (NCBI) 5715
RRIDAB_2880687
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO00233
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PSMD9 is a ubiquitous protein of eukaryotic cells and is a chaperon of the 26S proteasome complex, which degrades ubiquitinated proteins in eukaryotic cells and contributes to the degradation of intracellular proteins into antigenic peptides for antigen presentation by MHC class I cells. The 26S mammalian base sub-complex involves three distinct modules which have ATPase subunits distinctly associated to three chaperones, one of which is PSMD9 regulating the modules assembly. The PSMD9 ubiquitous regulatory role within the proteasome implies its potential pleiotropic effects within different physio-pathological systems. PSMD9 is known to form a stable subcomplex with PSMC3 and PSMC6, two of the AAA-ATPases, assisting in the assembly of the 20S and 19S particles to form the holo complex.

实验方案

Product Specific Protocols
WB protocol for PSMD9 antibody 26922-1-APDownload protocol
IHC protocol for PSMD9 antibody 26922-1-APDownload protocol
IF protocol for PSMD9 antibody 26922-1-APDownload protocol
IP protocol for PSMD9 antibody 26922-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols