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  • Featured Product
  • KD/KO Validated

PSMD9 Polyclonal antibody

PSMD9 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

Cat no : 26922-1-AP

Print datasheet

Synonyms

Rpn4, p27, 26S proteasome non-ATPase regulatory subunit 9

验证数据展示

  • WB analysis of A549 using 26922-1-AP

    WB analysis of A549 using 26922-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 26922-1-AP (PSMD9 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis of A549 using 26922-1-AP

    WB analysis of A549 using 26922-1-AP

    WB result of PSMD9 antibody (26922-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PSMD9 transfected A549 cells.

  • IHC staining of human breast cancer using 26922-1-AP

    IHC staining of human breast cancer using 26922-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 26922-1-AP (PSMD9 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human breast cancer using 26922-1-AP

    IHC staining of human breast cancer using 26922-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 26922-1-AP (PSMD9 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IP experiment of A549 using 26922-1-AP

    IP experiment of A549 using 26922-1-AP

    IP result of anti-PSMD9 (IP:26922-1-AP, 4ug; Detection:26922-1-AP 1:400) with A549 cells lysate 1330 ug.

  • IF Staining of U2OS using 26922-1-AP

    IF Staining of U2OS using 26922-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using PSMD9 antibody (26922-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • FC experiment of HeLa using 26922-1-AP

    FC experiment of HeLa using 26922-1-AP

    1x10^6 HeLa cells were intracellularly stained with 0.8 ug Anti-Human PSMD9 (26922-1-AP)(red), or 0.8 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive WB detected inA549 cells, MCF-7 cells, MDA-MB-453s cells, HepG2 cells, Jurkat cells, mouse spleen tissue
Positive IP detected inA549 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU2OS cells
Positive FC (Intra) detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26922-1-AP targets PSMD9 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description
Tested Reactivity human, mouse
Immunogen PSMD9 fusion protein Ag25638 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name proteasome (prosome, macropain) 26S subunit, non-ATPase, 9
Synonyms Rpn4, p27, 26S proteasome non-ATPase regulatory subunit 9
Calculated Molecular Weight 27 kDa
Observed Molecular Weight 25-30 kDa
GenBank Accession NumberBC004213
Gene Symbol PSMD9
Gene ID (NCBI) 5715
RRIDAB_2880687
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO00233
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PSMD9 is a ubiquitous protein of eukaryotic cells and is a chaperon of the 26S proteasome complex, which degrades ubiquitinated proteins in eukaryotic cells and contributes to the degradation of intracellular proteins into antigenic peptides for antigen presentation by MHC class I cells. The 26S mammalian base sub-complex involves three distinct modules which have ATPase subunits distinctly associated to three chaperones, one of which is PSMD9 regulating the modules assembly. The PSMD9 ubiquitous regulatory role within the proteasome implies its potential pleiotropic effects within different physio-pathological systems. PSMD9 is known to form a stable subcomplex with PSMC3 and PSMC6, two of the AAA-ATPases, assisting in the assembly of the 20S and 19S particles to form the holo complex.

实验方案

Product Specific Protocols
WB protocol for PSMD9 antibody 26922-1-APDownload protocol
IHC protocol for PSMD9 antibody 26922-1-APDownload protocol
IF protocol for PSMD9 antibody 26922-1-APDownload protocol
IP protocol for PSMD9 antibody 26922-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols