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验证数据展示

  • WB analysis using 67928-1-Ig (same clone as 67928-1-PBS)

    WB analysis using 67928-1-Ig (same clone as 67928-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67928-1-Ig (PSME1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 67928-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 67928-1-Ig (same clone as 67928-1-PBS)

    WB analysis of HeLa using 67928-1-Ig (same clone as 67928-1-PBS)

    WB result of PSME1 antibody (67928-1-Ig; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PSME1 transfected HeLa cells. This data was developed using the same antibody clone with 67928-1-PBS in a different storage buffer formulation.

  • IF Staining of MCF-7 using 67928-1-Ig (same clone as 67928-1-PBS)

    IF Staining of MCF-7 using 67928-1-Ig (same clone as 67928-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using PSME1 antibody (67928-1-Ig, Clone: 1F7E2 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67928-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

PSME1 Monoclonal antibody, PBS Only

PSME1 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 67928-1-PBS

产品说明书

CloneNo. 1F7E2

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, IF/ICC, Indirect ELISA

别名

REG-alpha, PA28A, Interferon gamma up-regulated I-5111 protein, IGUP I-5111, IFI5111

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy PSME1 Ready-To-Use IHC Kit. PSME1 primary antibody included.

产品信息

67928-1-PBS targets PSME1 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 PSME1 fusion protein Ag30549 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 proteasome (prosome, macropain) activator subunit 1 (PA28 alpha)
别名 REG-alpha, PA28A, Interferon gamma up-regulated I-5111 protein, IGUP I-5111, IFI5111
计算分子量 29 kDa
观测分子量29 kDa
GenBank蛋白编号BC007503
基因名称 PSME1
Gene ID (NCBI) 5720
RRIDAB_2918680
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ06323
储存缓冲液 PBS Only
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The principal function of the proteasome is targeted degradation of intracellular proteins. Activity of the 20S proteasome is controlled by regulatory complexes that bind to the ends of the cylindrical proteasome. 11S regulator (REG or PA28), is a complex of 28 kDa subunits that is thought to activate proteasomes toward the production of antigenic peptides. Human PSME1 and PSME2 genes encode the two proteasome activators PA28 alpha and beta, respectively, which have been implicated in antigen processing for loading class I MHC molecules. The PA28 activator complex enhances the generation of class I binding peptides by altering the cleavage pattern of the proteasome.