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  • Featured Product
  • KD/KO Validated

PSME1 Monoclonal antibody, PBS Only

PSME1 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1F7E2

Cat no : 67928-1-PBS

Print datasheet

Synonyms

REG-alpha, PA28A, Interferon gamma up-regulated I-5111 protein, IGUP I-5111, IFI5111

验证数据展示

  • WB analysis using 67928-1-Ig (same clone as 67928-1-PBS)

    WB analysis using 67928-1-Ig (same clone as 67928-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67928-1-Ig (PSME1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 67928-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 67928-1-Ig (same clone as 67928-1-PBS)

    WB analysis of HeLa using 67928-1-Ig (same clone as 67928-1-PBS)

    WB result of PSME1 antibody (67928-1-Ig; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PSME1 transfected HeLa cells. This data was developed using the same antibody clone with 67928-1-PBS in a different storage buffer formulation.

  • IF Staining of MCF-7 using 67928-1-Ig (same clone as 67928-1-PBS)

    IF Staining of MCF-7 using 67928-1-Ig (same clone as 67928-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using PSME1 antibody (67928-1-Ig, Clone: 1F7E2 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67928-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy PSME1 Ready-To-Use IHC Kit. PSME1 primary antibody included.

产品信息

67928-1-PBS targets PSME1 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IF/ICC, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen PSME1 fusion protein Ag30549 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name proteasome (prosome, macropain) activator subunit 1 (PA28 alpha)
Synonyms REG-alpha, PA28A, Interferon gamma up-regulated I-5111 protein, IGUP I-5111, IFI5111
Calculated Molecular Weight 29 kDa
Observed Molecular Weight29 kDa
GenBank Accession NumberBC007503
Gene Symbol PSME1
Gene ID (NCBI) 5720
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ06323
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The principal function of the proteasome is targeted degradation of intracellular proteins. Activity of the 20S proteasome is controlled by regulatory complexes that bind to the ends of the cylindrical proteasome. 11S regulator (REG or PA28), is a complex of 28 kDa subunits that is thought to activate proteasomes toward the production of antigenic peptides. Human PSME1 and PSME2 genes encode the two proteasome activators PA28 alpha and beta, respectively, which have been implicated in antigen processing for loading class I MHC molecules. The PA28 activator complex enhances the generation of class I binding peptides by altering the cleavage pattern of the proteasome.