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PSME3 Polyclonal antibody

PSME3 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 30009-1-AP

Print datasheet

Synonyms

Ki, Ki nuclear autoantigen, PA28 gamma, PA28G, PA28gamma, PSME3, REG GAMMA

验证数据展示

  • WB analysis of Caco-2 using 30009-1-AP

    WB analysis of Caco-2 using 30009-1-AP

    Caco-2 cells were subjected to SDS PAGE followed by western blot with 30009-1-AP (PSME3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inCaco-2 cells
Planning an IHC experiment? We recommend our IHCeasy PSME3 Ready-To-Use IHC Kit. PSME3 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30009-1-AP targets PSME3 in WB, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity Human
Immunogen PSME3 fusion protein Ag31969 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name proteasome (prosome, macropain) activator subunit 3 (PA28 gamma; Ki)
Synonyms Ki, Ki nuclear autoantigen, PA28 gamma, PA28G, PA28gamma, PSME3, REG GAMMA
Calculated Molecular Weight 30 kDa
Observed Molecular Weight30 kDa
GenBank Accession NumberBC001423
Gene Symbol PSME3
Gene ID (NCBI) 10197
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP61289
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PSME3 gene encodes proteasome activator complex subunit 3, which is also called REG-gamma or PA28-gamma. REG-gamma activates the trypsin-like catalytic subunit of the proteasome thus is a proteasome regulator. MDM2-TP53 interaction which promotes ubiquitination- and MDM2-dependent proteasomal degradation of TP53, may be promoted by REG-gamma resulting in inhibited apoptosis after DNA damage. REG-gamma may also be involved in cell cycle regulation.

实验方案

Product Specific Protocols
WB protocol for PSME3 antibody 30009-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols