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PSME4 Polyclonal antibody
PSME4 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF/ICC, IP, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, HepG2 cells |
| Positive IP detected in | HEK-293 cells |
| Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells, Hela cells |
Planning an IHC experiment? We recommend our IHCeasy PSME4 Ready-To-Use IHC Kit. PSME4 primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
18799-1-AP targets PSME4 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, IHC, IF/ICC, IP, ELISA Application Description |
| Cited Applications | WB, IHC, CoIP |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Immunogen | Peptide 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | proteasome (prosome, macropain) activator subunit 4 |
| Synonyms | PSME 4, KIAA0077 |
| Calculated Molecular Weight | 211 kDa |
| Observed Molecular Weight | 140 kDa |
| GenBank Accession Number | BC113668 |
| Gene Symbol | PSME4 |
| Gene ID (NCBI) | 23198 |
| RRID | AB_10598020 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q14997 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PSME4, like PSME1, 2, and 3, encodes proteasome activator complex subunit 4 also named proteasome activator 200 kDa (PA200). PA200 activates proteasomal cleavage of peptides in an energy-independent manner. After exposure of cells to ionizing radiation, PA200 forms nuclear foci and enhances proteasome activity independent of the stage of cell cycle arrest. PA200 also may be involved in spermatogenesis. The antibody is expected to recognize all the 4 isoforms of PSME4 but appears to mainly detect isoform 2 of 140-kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PSME4 antibody 18799-1-AP | Download protocol |
| IHC protocol for PSME4 antibody 18799-1-AP | Download protocol |
| IF protocol for PSME4 antibody 18799-1-AP | Download protocol |
| IP protocol for PSME4 antibody 18799-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
EBioMedicine CRIP1 involves the pathogenesis of multiple myeloma via dual-regulation of proteasome and autophagy | ||
EBioMedicine Indirubin-3'-monoxime acts as proteasome inhibitor: Therapeutic application in multiple myeloma.
| ||
Int J Gen Med PSME4 Activates mTOR Signaling and Promotes the Malignant Progression of Hepatocellular Carcinoma.
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