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验证数据展示

  • WB analysis using 10335-1-AP

    WB analysis using 10335-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10335-1-AP (PSMG1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human stomach cancer using 10335-1-AP

    IHC staining of human stomach cancer using 10335-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 10335-1-AP (PSMG1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PSMG1 antibody included
  • IHC staining of human stomach cancer using 10335-1-AP

    IHC staining of human stomach cancer using 10335-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 10335-1-AP (PSMG1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PSMG1 antibody included

PSMG1 Polyclonal antibody

PSMG1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 10335-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

C21 LRP, C21LRP, DSCR2, LRPC21, PAC 1, PAC1, PSMG1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293T cells, HepG2 cells, Jurkat cells
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy PSMG1 Ready-To-Use IHC Kit. PSMG1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

10335-1-AP targets PSMG1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原 PSMG1 fusion protein Ag0303 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 proteasome (prosome, macropain) assembly chaperone 1
别名 C21 LRP, C21LRP, DSCR2, LRPC21, PAC 1, PAC1, PSMG1
计算分子量 288 aa, 33 kDa
观测分子量 30-33 kDa
GenBank蛋白编号BC003619
基因名称 PSMG1
Gene ID (NCBI) 8624
RRIDAB_2877733
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO95456
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PSMG1 antibody 10335-1-APDownload protocol
IHC protocol for PSMG1 antibody 10335-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Stem Cell Res Ther

Melatonin induces the rejuvenation of long-term ex vivo expanded periodontal ligament stem cells by modulating the autophagic process.

Authors - Yi-Zhou Tan
View Article

Oncol Rep

Association of p53/p21 expression and cigarette smoking with tumor progression and poor prognosis in non-small cell lung cancer patients.

Authors - Deyao Xie
View Article