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验证数据展示

  • WB analysis using 10959-1-AP

    WB analysis using 10959-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10959-1-AP (PSMG2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

PSMG2 Polyclonal antibody

PSMG2 Polyclonal Antibody for WB, ELISA
Cat No. 10959-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, ELISA

别名

PAC2, MDS003, HsT1707, Hepatocellular carcinoma-susceptibility protein 3, HCCA3

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, Jurkat cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10959-1-AP targets PSMG2 in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原 PSMG2 fusion protein Ag1400 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 proteasome (prosome, macropain) assembly chaperone 2
别名 PAC2, MDS003, HsT1707, Hepatocellular carcinoma-susceptibility protein 3, HCCA3
计算分子量 29 kDa
观测分子量 29 kDa
GenBank蛋白编号BC013356
基因名称 PSMG2
Gene ID (NCBI) 56984
RRIDAB_2171454
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ969U7
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Proteasome assembly chaperone 2 (PSMG2), also named as Hepatocellular carcinoma-susceptibility protein 3, Tumor necrosis factor superfamily member 5-induced protein 1. It has a function as a chaperone protein which promotes assembly of the 20S proteasome as part of a heterodimer with PSMG1. The PSMG1-PSMG2 heterodimer binds to the PSMA5 and PSMA7 proteasome subunits, promotes assembly of the proteasome alpha subunits into the heteroheptameric alpha ring and prevents alpha ring dimerization.It is widely expressed with highest levels in lung, brain and colon,moderately expressed in muscle, stomach, spleen and heart and weakly expressed in small intestine, pancreas and liver,highly expressed in hepatocellular carcinomas with low levels in surrounding liver tissue. Molecular mass of PSMG2 is 29kD.

实验方案

Product Specific Protocols
WB protocol for PSMG2 antibody 10959-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols