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  • Featured Product
  • KD/KO Validated

PTBP1 Monoclonal antibody, PBS Only

PTBP1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3D6D8

Cat no : 67462-1-PBS

Print datasheet

Synonyms

HNRNP I, HNRNPI, HNRPI, pPTB, PTB, PTB 1, PTB T, PTB2, PTB3, PTB4, PTBP1

验证数据展示

  • WB analysis using 67462-1-Ig (same clone as 67462-1-PBS)

    WB analysis using 67462-1-Ig (same clone as 67462-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67462-1-Ig (PTBP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67462-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 67462-1-Ig (same clone as 67462-1-PBS)

    IHC staining of human colon cancer using 67462-1-Ig (same clone as 67462-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67462-1-Ig (PTBP1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67462-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PTBP1 antibody included
  • IF Staining of A375 using 67462-1-Ig (same clone as 67462-1-PBS)

    IF Staining of A375 using 67462-1-Ig (same clone as 67462-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A375 cells using 67462-1-Ig (PTBP1 antibody), at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67462-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 67462-1-Ig (same clone as 67462-1-PBS)

    WB analysis of HeLa using 67462-1-Ig (same clone as 67462-1-PBS)

    WB result of PTBP1 antibody (67462-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PTBP1 transfected HeLa cells. This data was developed using the same antibody clone with 67462-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy PTBP1 Ready-To-Use IHC Kit. PTBP1 primary antibody included.

产品信息

67462-1-PBS targets PTBP1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity Human, mouse, rat
Immunogen PTBP1 fusion protein Ag28404 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name polypyrimidine tract binding protein 1
Synonyms HNRNP I, HNRNPI, HNRPI, pPTB, PTB, PTB 1, PTB T, PTB2, PTB3, PTB4, PTBP1
Calculated Molecular Weight 60 kDa
Observed Molecular Weight 60 kDa
GenBank Accession NumberBC013694
Gene Symbol PTBP1
Gene ID (NCBI) 5725
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP26599
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Polypyrimidine tract-binding protein (PTBP1), also known as heterogeneous nuclear ribonucleoprotein type I (hnRNP I), is a nuclear protein that binds pre-mRNAs in specific regions of the hnRNA-protein complexes sensitive to micrococcal nuclease. It involves in pre-mRNA splicing and the regulation of alternative splicing events. PTBP1 promotes RNA looping when bound to two separate polypyrimidine tracts in the same pre-mRNA, and promotes the binding of U2 snRNP to pre-mRNA. Besides, it can cooperate with RAVER1 to modulate switching between mutually exclusive exons during maturation of the TPM1 pre-mRNA, and represses the splicing of MAPT/Tau exon 10.