验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells, human brain tissue, mouse liver tissue, rat liver tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 3 publications below |
产品信息
55091-1-AP targets PTCH2 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | patched homolog 2 (Drosophila) |
| 别名 | UNQ560/PRO1121/PRO57079, PTC2, Protein patched homolog 2, patched homolog 2 (Drosophila), Patched 2 |
| 计算分子量 | 131 kDa |
| 观测分子量 | 131-140 kDa |
| GenBank蛋白编号 | NM_003738 |
| 基因名称 | PTCH2 |
| Gene ID (NCBI) | 8643 |
| RRID | AB_10858392 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9Y6C5 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PTCH1 (patched homolog 1) and PTCH2 are twelve-pass transmembrane proteins that act receptors of Hedgehog signaling pathway (PMID: 8906794; 9811851). Signals of Hedgehog family proteins (SHH, IHH and DHH in humans) are transduced through Patched family receptors (PTCH1 and PTCH2) and Smoothened (SMO) to GLI family transcription factors. Hedgehog pathway plays a critical role in embryonic development and tissue homeostasis, and its dysregulation has been implicated in many human diseases including congenital disorder and cancer (PMID: 23719536; 23532857). PTCH2 may function as a tumor suppressor in the Hedgehog signaling pathway. Alterations in PTCH2 gene have been associated with nevoid basal cell carcinoma syndrome, basal cell carcinoma, medulloblastoma, and susceptibility to congenital macrostomia. This antibody is specific to PTCH2.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PTCH2 antibody 55091-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Exp Mol Med Lentivirus-mediated silencing of the PTC1 and PTC2 genes promotes recovery from spinal cord injury by activating the Hedgehog signaling pathway in a rat model. | ||
iScience 4-(Methylnitrosamino)-1-(3-pyridyl)-1-butanone provokes progression from chronic pancreatitis to pancreatic intraepithelial neoplasia. | ||
Cell Res SCUBE2 mediates bone metastasis of luminal breast cancer by modulating immune-suppressive osteoblastic niches |
